RGD Reference Report - Role of PDGF B-chain and PDGF receptors in rat tubular regeneration after acute injury. - Rat Genome Database

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Role of PDGF B-chain and PDGF receptors in rat tubular regeneration after acute injury.

Authors: Nakagawa, T  Sasahara, M  Haneda, M  Kataoka, H  Nakagawa, H  Yagi, M  Kikkawa, R  Hazama, F 
Citation: Nakagawa T, etal., Am J Pathol. 1999 Nov;155(5):1689-99.
RGD ID: 8554477
Pubmed: PMID:10550325   (View Abstract at PubMed)
PMCID: PMC1866980   (View Article at PubMed Central)
DOI: DOI:10.1016/S0002-9440(10)65484-3   (Journal Full-text)

Various polypeptide growth factors are generally considered to be involved in the regulation of the nephrogenic process both after acute renal injury and during renal development. Because platelet-derived growth factor B-chain (PDGF-B) has been reported to be expressed in immature tubulus of the developing kidney, PDGF-B could play a role in the process of tubulogenesis. We examined the expression of PDGF-B and PDGF receptors alpha and beta and their localization in kidneys after ischemia/reperfusion injury. The mRNA expressions of PDGF-B, PDGFR-alpha, and PDGFR-beta were enhanced after injury. In the immunohistochemical analysis and/or in situ hybridization, PDGF-B and PDGFR-alpha, beta were expressed after reperfusion in the S3 segment of the proximal tubuli, where they were not expressed normally. The expressions of proliferating cell nuclear antigen and vimentin were concomitantly observed with PDGF-B and PDGFRs in the tubular cells of injured S3 segment at 48 hours after injury. Next, the inhibition of the PDGF-B/PDGFRs axis with either Trapidil or Ki6896, which was found to inhibit the phosphorylation of PDGFR-beta selectively, resulted in a rise of serum creatinine, higher mortality rate, abnormal regenerating process, and suppressed proliferation of tubular epithelial cells. These findings suggest that the PDGF-B/PDGFRs axis is involved in the proliferation of injured tubular cells and plays an important role in the regeneration of tubular cells from acute ischemic injury.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Kidney Reperfusion Injury  ISOPdgfb (Rattus norvegicus)8554477; 8554477mRNA more ...RGD 
Kidney Reperfusion Injury  ISOPdgfra (Rattus norvegicus)8554477; 8554477mRNA:increased expression:kidneyRGD 
Kidney Reperfusion Injury  ISOPdgfrb (Rattus norvegicus)8554477; 8554477mRNA:increased expression:kidneyRGD 
Kidney Reperfusion Injury  IEP 8554477mRNA more ...RGD 
Kidney Reperfusion Injury  IEP 8554477; 8554477mRNA:increased expression:kidneyRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane located_inIDA 8554477PMID:10550325UniProt 
basolateral plasma membrane located_inIDA 8554477PMID:10550325UniProt 
cytoplasm located_inIDA 8554477PMID:10550325UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfb  (platelet derived growth factor subunit B)
Pdgfra  (platelet derived growth factor receptor alpha)
Pdgfrb  (platelet derived growth factor receptor beta)

Genes (Mus musculus)
Pdgfb  (platelet derived growth factor, B polypeptide)
Pdgfra  (platelet derived growth factor receptor, alpha polypeptide)
Pdgfrb  (platelet derived growth factor receptor, beta polypeptide)

Genes (Homo sapiens)
PDGFB  (platelet derived growth factor subunit B)
PDGFRA  (platelet derived growth factor receptor alpha)
PDGFRB  (platelet derived growth factor receptor beta)


Additional Information