RGD Reference Report - Evidence for a functional nitric oxide synthase system in brown adipocyte nucleus. - Rat Genome Database

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Evidence for a functional nitric oxide synthase system in brown adipocyte nucleus.

Authors: Giordano, A  Tonello, C  Bulbarelli, A  Cozzi, V  Cinti, S  Carruba, MO  Nisoli, E 
Citation: Giordano A, etal., FEBS Lett. 2002 Mar 13;514(2-3):135-40.
RGD ID: 8554470
Pubmed: PMID:11943139   (View Abstract at PubMed)

The intracellular localization and activity of the nitric oxide synthase (NOS) isoforms were investigated in rat brown adipocytes. Immunohistochemistry showed cytoplasmic and nuclear staining for the endothelial NOS (eNOS) and inducible NOS (iNOS) isoforms; accordingly, anti-L-citrulline antibody, a marker of NOS activity, immunostained both the cytoplasm and the nucleus. The presence of metabolically active NOS in the nucleus was further confirmed by immunoblotting analyses of subcellular fractions of homogenates from cultured brown adipocytes and by measurements of NOS activity in the cytosol and nucleus. Sympathetic stimulation in vivo (i.e. cold exposure or beta(3)-adrenergic agonist treatment) and in vitro (i.e. noradrenaline treatment of cultured cells) significantly increased both cytosolic and nuclear eNOS and iNOS expression and activities. By contrast, the number of iNOS-positive, but not eNOS-positive, nuclei was significantly lower in the functionally impaired brown fat of genetically obese Zucker fa/fa rats. These data suggest the existence of a noradrenaline-modulated functional NOS system in the nucleus of brown adipocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoplasm located_inIDA 8554470; 8554470PMID:11943139BHF-UCL 
nucleus located_inIDA 8554470; 8554470PMID:11943139BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Nos2  (nitric oxide synthase 2)
Nos3  (nitric oxide synthase 3)


Additional Information