RGD Reference Report - Membrane-embedded C-terminal segment of rat mitochondrial TOM40 constitutes protein-conducting pore with enriched beta-structure. - Rat Genome Database

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Membrane-embedded C-terminal segment of rat mitochondrial TOM40 constitutes protein-conducting pore with enriched beta-structure.

Authors: Suzuki, H  Kadowaki, T  Maeda, M  Sasaki, H  Nabekura, J  Sakaguchi, M  Mihara, K 
Citation: Suzuki H, etal., J Biol Chem. 2004 Nov 26;279(48):50619-29. Epub 2004 Sep 3.
RGD ID: 8554396
Pubmed: PMID:15347672   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M408604200   (Journal Full-text)

TOM40 is the central component of the preprotein translocase of the mitochondrial outer membrane (TOM complex). We purified recombinant rat TOM40 (rTOM40), which was refolded in Brij35 after solubilization from inclusion bodies by guanidine HCl. rTOM40 (i) consisted of a 63% beta-sheet structure and (ii) bound a matrix-targeted preprotein with high affinity and partially translocated it into the rTOM40 pore. This partial translocation was inhibited by stabilization of the mature domain of the precursor. (iii) rTOM40 bound preprotein initially through ionic interactions, followed by salt-resistant non-ionic interactions, and (iv) exhibited presequence-sensitive, cation-specific channel activity in reconstituted liposomes. Based on the domain structure of rTOM40 deduced by protease treatment, we purified the elastase-resistant and membrane-embedded C-terminal segment (rTOM40(DeltaN165)) as a recombinant protein with 62% beta-structure that exhibited properties comparable with those of full-size rTOM40. We concluded that the membrane-embedded C-terminal half of rTOM40 constitutes the preprotein recognition domain with an enriched beta-structure, which forms the preprotein conducting pore containing a salt-sensitive cis-binding site and a salt-resistant trans-binding site.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein targeting to mitochondrion involved_inIMP 8554396PMID:15347672UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
monoatomic cation channel activity  IDA 8554396 RGD 
preprotein binding  IDA 8554396 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tomm40  (translocase of outer mitochondrial membrane 40)


Additional Information