RGD Reference Report - Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin. - Rat Genome Database

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Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin.

Authors: Schumacher, MA  Rivard, AF  Bachinger, HP  Adelman, JP 
Citation: Schumacher MA, etal., Nature. 2001 Apr 26;410(6832):1120-4.
RGD ID: 8554362
Pubmed: PMID:11323678   (View Abstract at PubMed)
DOI: DOI:10.1038/35074145   (Journal Full-text)

Small-conductance Ca2+-activated K+ channels (SK channels) are independent of voltage and gated solely by intracellular Ca2+. These membrane channels are heteromeric complexes that comprise pore-forming alpha-subunits and the Ca2+-binding protein calmodulin (CaM). CaM binds to the SK channel through the CaM-binding domain (CaMBD), which is located in an intracellular region of the alpha-subunit immediately carboxy-terminal to the pore. Channel opening is triggered when Ca2+ binds the EF hands in the N-lobe of CaM. Here we report the 1.60 A crystal structure of the SK channel CaMBD/Ca2+/CaM complex. The CaMBD forms an elongated dimer with a CaM molecule bound at each end; each CaM wraps around three alpha-helices, two from one CaMBD subunit and one from the other. As only the CaM N-lobe has bound Ca2+, the structure provides a view of both calcium-dependent and -independent CaM/protein interactions. Together with biochemical data, the structure suggests a possible gating mechanism for the SK channel.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
potassium ion transport involved_inIDA 8554362PMID:11323678UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
membrane located_inIDA 8554362PMID:11323678UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
small conductance calcium-activated potassium channel activity enablesIDA 8554362PMID:11323678UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Kcnn2  (potassium calcium-activated channel subfamily N member 2)

Objects referenced in this article
Gene Calm1 calmodulin 1 Rattus norvegicus
Gene Calm2 calmodulin 2 Rattus norvegicus
Gene Calm3 calmodulin 3 Rattus norvegicus

Additional Information