RGD Reference Report - ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. - Rat Genome Database

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ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation.

Authors: Gille, H  Kortenjann, M  Thomae, O  Moomaw, C  Slaughter, C  Cobb, MH  Shaw, PE 
Citation: Gille H, etal., EMBO J. 1995 Mar 1;14(5):951-62.
RGD ID: 8554315
Pubmed: PMID:7889942   (View Abstract at PubMed)
PMCID: PMC398167   (View Article at PubMed Central)

Induction of the human c-fos proto-oncogene by mitogens depends on the formation of a ternary complex by p62TCF with the serum response factor (SRF) and the serum response element (SRE). We demonstrate that Elk-1, a protein closely related to p62TCF in function, is a nuclear target of two members of the MAP kinase family, ERK1 and ERK2. Phosphorylation of Elk-1 increases the yield of ternary complex in vitro. At least five residues in the C-terminal domain of Elk-1 are phosphorylated upon growth factor stimulation of NIH3T3 cells. These residues are also phosphorylated by purified ERK1 in vitro, as determined by a combination of phosphopeptide sequencing and 2-D peptide mapping. Conversion of two of these phospho-acceptor sites to alanine impairs the formation of ternary complexes by the resulting Elk-1 proteins. Removal of these serine residues also drastically diminishes activation of the c-fos promoter in epidermal growth factor-treated cells. Analogous mutations at other sites impair activation to a lesser extent without affecting ternary complex formation in vitro. Our results indicate that phosphorylation regulates ternary complex formation by Elk-1, which is a prerequisite for the manifestation of its transactivation potential at the c-fos SRE.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex assembly involved_inIMP 8554315PMID:7889942UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nucleoplasm located_inIDA 8554315; 8554315PMID:7889942UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
MAP kinase activity enablesIDA 8554315; 8554315PMID:7889942UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Mapk1  (mitogen activated protein kinase 1)
Mapk3  (mitogen activated protein kinase 3)


Additional Information