RGD Reference Report - AP180 and CALM in the developing hippocampus: expression at the nascent synapse and localization to trafficking organelles. - Rat Genome Database

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AP180 and CALM in the developing hippocampus: expression at the nascent synapse and localization to trafficking organelles.

Authors: Petralia, RS  Yao, PJ 
Citation: Petralia RS and Yao PJ, J Comp Neurol. 2007 Sep 20;504(3):314-27.
RGD ID: 8554206
Pubmed: PMID:17640037   (View Abstract at PubMed)
DOI: DOI:10.1002/cne.21454   (Journal Full-text)

Genetic and biochemical evidence has established that clathrin assembly protein AP180 is required for the proper assembly of synaptic vesicles via clathrin-mediated endocytosis. The assembly protein CALM, the ubiquitously expressed homolog of AP180, also regulates the formation of clathrin-coated vesicles. In this study we found high expression levels of AP180 and CALM in hippocampal tissues as early as embryonic day 18, before the expression of synaptophysin. We also used immunoelectron microscopy to establish the distribution of AP180 and CALM in the developing hippocampal synapses. We found AP180 and CALM in synapses at all developmental stages and in nonsynaptic growing processes. In addition to localization on the plasma membrane and clathrin-coated vesicles that originated from the plasma membrane, we also report the presence of AP180 and CALM on other types of membrane structures. Our observations link AP180 and CALM to multiple vesicular organelles and raise the possibility that these proteins may play additional roles in developing neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
clathrin-coated vesicle located_inIDA 8554206PMID:17640037BHF-UCL 
postsynaptic density located_inIDA 8554206; 8554206PMID:17640037BHF-UCL 
postsynaptic membrane located_inIDA 8554206; 8554206PMID:17640037BHF-UCL 
presynaptic membrane located_inIDA 8554206; 8554206PMID:17640037BHF-UCL 
synaptic vesicle located_inIDA 8554206; 8554206PMID:17640037BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Picalm  (phosphatidylinositol binding clathrin assembly protein)
Snap91  (synaptosome associated protein 91)


Additional Information