RGD Reference Report - Distinct molecular mechanisms and divergent endocytotic pathways of AMPA receptor internalization. - Rat Genome Database

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Distinct molecular mechanisms and divergent endocytotic pathways of AMPA receptor internalization.

Authors: Lin, JW  Ju, W  Foster, K  Lee, SH  Ahmadian, G  Wyszynski, M  Wang, YT  Sheng, M 
Citation: Lin JW, etal., Nat Neurosci. 2000 Dec;3(12):1282-90.
RGD ID: 8554168
Pubmed: PMID:11100149   (View Abstract at PubMed)
DOI: DOI:10.1038/81814   (Journal Full-text)

Internalization of postsynaptic AMPA receptors depresses excitatory transmission, but the underlying dynamics and mechanisms of this process are unclear. Using immunofluorescence and surface biotinylation, we characterized and quantified basal and regulated AMPA receptor endocytosis in cultured hippocampal neurons, in response to synaptic activity, AMPA and insulin. AMPA-induced AMPA receptor internalization is mediated in part by secondary activation of voltage-dependent calcium channels, and in part by ligand binding independent of receptor activation. Although both require dynamin, insulin- and AMPA-induced AMPA receptor internalization are differentially dependent on protein phosphatases and sequence determinants within the cytoplasmic tails of GluR1 and GluR2 subunits. AMPA receptors internalized in response to AMPA stimulation enter a recycling endosome system, whereas those internalized in response to insulin diverge into a distinct compartment. Thus, the molecular mechanisms and intracellular sorting of AMPA receptors are diverse, and depend on the internalizing stimulus.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
receptor internalization involved_inIMP 8554168PMID:11100149UniProt 
receptor internalization involved_inIDA 8554168; 8554168PMID:11100149UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendritic shaft located_inIDA 8554168; 8554168PMID:11100149UniProt 
early endosome located_inIDA 8554168PMID:11100149UniProt 
neuronal cell body located_inIDA 8554168; 8554168PMID:11100149UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Dnm1  (dynamin 1)
Gria1  (glutamate ionotropic receptor AMPA type subunit 1)
Gria2  (glutamate ionotropic receptor AMPA type subunit 2)


Additional Information