RGD Reference Report - The specificity of SNARE pairing in biological membranes is mediated by both proof-reading and spatial segregation. - Rat Genome Database

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The specificity of SNARE pairing in biological membranes is mediated by both proof-reading and spatial segregation.

Authors: Bethani, I  Lang, T  Geumann, U  Sieber, JJ  Jahn, R  Rizzoli, SO 
Citation: Bethani I, etal., EMBO J. 2007 Sep 5;26(17):3981-92. Epub 2007 Aug 23.
RGD ID: 8554124
Pubmed: PMID:17717530   (View Abstract at PubMed)
PMCID: PMC1994121   (View Article at PubMed Central)
DOI: DOI:10.1038/sj.emboj.7601820   (Journal Full-text)

Soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins mediate organelle fusion in the secretory pathway. Different fusion steps are catalyzed by specific sets of SNARE proteins. Here we have used the SNAREs mediating the fusion of early endosomes and exocytosis, respectively, to investigate how pairing specificity is achieved. Although both sets of SNAREs promiscuously assemble in vitro, there is no functional crosstalk. We now show that they not only colocalize to overlapping microdomains in the membrane of early endosomes of neuroendocrine cells, but also form cis-complexes promiscuously, with the proportion of the different complexes being primarily dependent on mass action. Addition of soluble SNARE molecules onto native membranes revealed preference for cognate SNAREs. Furthermore, we found that SNAREs are laterally segregated at endosome contact sites, with the exocytotic synaptobrevin being depleted. We conclude that specificity in endosome fusion is mediated by the following two synergistically operating mechanisms: (i) preference for the cognate SNARE in 'trans' interactions and (ii) lateral segregation of SNAREs, leading to relative enrichment of the cognate ones at the prospective fusion sites.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Snap25  (synaptosome associated protein 25)
Stx12  (syntaxin 12)
Stx1a  (syntaxin 1A)
Vamp1  (vesicle-associated membrane protein 1)


Additional Information