RGD Reference Report - RAB13 regulates Sertoli cell permeability barrier dynamics through protein kinase A. - Rat Genome Database

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RAB13 regulates Sertoli cell permeability barrier dynamics through protein kinase A.

Authors: Su, W  Liu, X 
Citation: Su W and Liu X, J Mol Endocrinol. 2013 Apr 12;50(3):305-18. doi: 10.1530/JME-13-0011. Print 2013 Jun.
RGD ID: 8554106
Pubmed: PMID:23419316   (View Abstract at PubMed)
DOI: DOI:10.1530/JME-13-0011   (Journal Full-text)

In mammalian testes, the blood-testis barrier (BTB), created by specialized junctions between Sertoli cells near the basement membrane of the seminiferous epithelium, provides an indispensable immune-privileged microenvironment for spermatid development. However, the BTB must experience restructuring during the epithelial cycle to facilitate the transit of preleptotene spermatocytes upon the testosterone-induced new TJ fibrils forming behind these cells, which is intimately related to the extensive dynamics of junction protein complexes between Sertoli cells. As key regulators of protein traffic, Rab GTPases participate in delivery of proteins between distinct cellular sites and cross talk with proteins that constitute tight junction and adherens junction. Using primarily cultured Sertoli cells in vitro with an established tight junction permeability barrier that mimics the BTB in vivo, RAB13 was shown to decrease during the testosterone-induced TJ integrity enhancement, accompanied with an increment in protein kinase A (PKA) activity. Furthermore, knockdown of Rab13 was found to resemble the effect of testosterone on Sertoli cell TJ permeability by reinforcing filamentous actin and occludin distribution at the cell-cell interface and promoting the direct interaction between ZO-1 and occludin. Interestingly, the effects of testosterone and Rab13 knockdown on Sertoli cell epithelium were revealed to be antagonized by PKA activity inhibition. In summary, RAB13 serves as a regulatory component in the assembly and restructuring of the TJ fibrils between adjacent Sertoli cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cortical actin cytoskeleton organization involved_inIMP 8554106PMID:23419316UniProt 
establishment of Sertoli cell barrier involved_inIMP 8554106PMID:23419316UniProt 
protein kinase A signaling involved_inIMP 8554106PMID:23419316UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein kinase A catalytic subunit binding enablesIPIUniProtKB:P277918554106PMID:23419316UniProt 
small GTPase binding enablesIPIUniProtKB:P352868554106PMID:23419316UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Prkaca  (protein kinase cAMP-activated catalytic subunit alpha)
Rab13  (RAB13, member RAS oncogene family)


Additional Information