RGD Reference Report - Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes. - Rat Genome Database

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Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes.

Authors: Pakhomova, S  Luka, Z  Grohmann, S  Wagner, C  Newcomer, ME 
Citation: Pakhomova S, etal., Proteins. 2004 Nov 1;57(2):331-7.
RGD ID: 8553948
Pubmed: PMID:15340920   (View Abstract at PubMed)
DOI: DOI:10.1002/prot.20209   (Journal Full-text)

Glycine N-methyltransferases (GNMTs) from three mammalian sources were compared with respect to their crystal structures and kinetic parameters. The crystal structure for the rat enzyme was published previously. Human and mouse GNMT were expressed in Escherichia coli in order to determine their crystal structures. Mouse GNMT was crystallized in two crystal forms, a monoclinic form and a tetragonal form. Comparison of the three structures reveals subtle differences, which may relate to the different kinetic properties of the enzymes. The flexible character of several loops surrounding the active site, along with an analysis of the active site boundaries, indicates that the observed conformations of human and mouse GNMTs are more open than that of the rat enzyme. There is an increase in kcat when going from rat to mouse to human, suggesting a correlation with the increased flexibility of some structural elements of the respective enzymes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein homotetramerization involved_inIPIUniProtKB:P132558553948PMID:15340920UniProt 
S-adenosylmethionine metabolic process involved_inIDA 8553948PMID:15340920UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glycine binding enablesIDA 8553948PMID:15340920UniProt 
glycine N-methyltransferase activity enablesIDA 8553948PMID:15340920UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Gnmt  (glycine N-methyltransferase)


Additional Information