RGD Reference Report - SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release. - Rat Genome Database

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SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release.

Authors: Inoue, E  Mochida, S  Takagi, H  Higa, S  Deguchi-Tawarada, M  Takao-Rikitsu, E  Inoue, M  Yao, I  Takeuchi, K  Kitajima, I  Setou, M  Ohtsuka, T  Takai, Y 
Citation: Inoue E, etal., Neuron. 2006 Apr 20;50(2):261-75.
RGD ID: 8553864
Pubmed: PMID:16630837   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuron.2006.03.018   (Journal Full-text)

A serine/threonine kinase SAD-1 in C. elegans regulates synapse development. We report here the isolation and characterization of mammalian orthologs of SAD-1, named SAD-A and SAD-B, which are specifically expressed in the brain. SAD-B is associated with synaptic vesicles and, like the active zone proteins CAST and Bassoon, is tightly associated with the presynaptic cytomatrix in nerve terminals. A short conserved region (SCR) in the COOH-terminus is required for the synaptic localization of SAD-B. Overexpression of SAD-B in cultured rat hippocampal neurons significantly increases the frequency of miniature excitatory postsynaptic current but not its amplitude. Introduction of SCR into presynaptic superior cervical ganglion neurons in culture significantly inhibits evoked synaptic transmission. Moreover, SCR decreases the size of the readily releasable pool measured by applying hypertonic sucrose. Furthermore, SAD-B phosphorylates the active zone protein RIM1 but not Munc13-1. These results suggest that mammalian SAD kinase presynaptically regulates neurotransmitter release.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cholinergic synapse is_active_inIMP 8553864PMID:16630837SynGO 
cholinergic synapse is_active_inIEP 8553864PMID:16630837SynGO 
cholinergic synapse is_active_inIDA 8553864PMID:16630837SynGO 
postsynaptic density is_active_inIEP 8553864PMID:16630837SynGO 
postsynaptic density is_active_inIDA 8553864PMID:16630837SynGO 
synaptic vesicle located_inIDA 8553864PMID:16630837UniProt 
synaptic vesicle is_active_inIEP 8553864PMID:16630837SynGO 
synaptic vesicle is_active_inIDA 8553864PMID:16630837SynGO 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Brsk1  (BR serine/threonine kinase 1)
Rims1  (regulating synaptic membrane exocytosis 1)


Additional Information