RGD Reference Report - Amphiphysin II (SH3P9; BIN1), a member of the amphiphysin/Rvs family, is concentrated in the cortical cytomatrix of axon initial segments and nodes of ranvier in brain and around T tubules in skeletal muscle. - Rat Genome Database

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Amphiphysin II (SH3P9; BIN1), a member of the amphiphysin/Rvs family, is concentrated in the cortical cytomatrix of axon initial segments and nodes of ranvier in brain and around T tubules in skeletal muscle.

Authors: Butler, MH  David, C  Ochoa, GC  Freyberg, Z  Daniell, L  Grabs, D  Cremona, O  De Camilli, P 
Citation: Butler MH, etal., J Cell Biol. 1997 Jun 16;137(6):1355-67.
RGD ID: 8553847
Pubmed: PMID:9182667   (View Abstract at PubMed)
PMCID: PMC2132527   (View Article at PubMed Central)

Amphiphysin (amphiphysin I), a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals, where it has a putative role in endocytosis. The yeast homologue of amphiphysin, Rvs167, has pleiotropic functions, including a role in endocytosis and in actin dynamics, suggesting that amphiphysin may also be implicated in the function of the presynaptic actin cytoskeleton. We report here the characterization of a second mammalian amphiphysin gene, amphiphysin II (SH3P9; BIN1), which encodes products primarily expressed in skeletal muscle and brain, as differentially spliced isoforms. In skeletal muscle, amphiphysin II is concentrated around T tubules, while in brain it is concentrated in the cytomatrix beneath the plasmamembrane of axon initial segments and nodes of Ranvier. In both these locations, amphiphysin II is colocalized with splice variants of ankyrin3 (ankyrinG), a component of the actin cytomatrix. In the same regions, the presence of clathrin has been reported. These findings support the hypothesis that, even in mammalian cells, amphiphysin/Rvs family members have a role both in endocytosis and in actin function and suggest that distinct amphiphysin isoforms contribute to define distinct domains of the cortical cytoplasm. Since amphiphysin II (BIN1) was reported to interact with Myc, it may also be implicated in a signaling pathway linking the cortical cytoplasm to nuclear function.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon initial segment located_inIDA 8553847PMID:9182667Alzheimers_University_of_Toronto 
I band located_inIDA 8553847PMID:9182667Alzheimers_University_of_Toronto 
node of Ranvier located_inIDA 8553847PMID:9182667Alzheimers_University_of_Toronto 
T-tubule located_inIDA 8553847PMID:9182667Alzheimers_University_of_Toronto 
Z disc located_inIDA 8553847PMID:9182667Alzheimers_University_of_Toronto 

Objects Annotated

Genes (Rattus norvegicus)
Bin1  (bridging integrator 1)


Additional Information