RGD Reference Report - Clathrin is required for the function of the mitotic spindle. - Rat Genome Database

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Clathrin is required for the function of the mitotic spindle.

Authors: Royle, SJ  Bright, NA  Lagnado, L 
Citation: Royle SJ, etal., Nature. 2005 Apr 28;434(7037):1152-7.
RGD ID: 8553642
Pubmed: PMID:15858577   (View Abstract at PubMed)
PMCID: PMC3492753   (View Article at PubMed Central)
DOI: DOI:10.1038/nature03502   (Journal Full-text)

Clathrin has an established function in the generation of vesicles that transfer membrane and proteins around the cell. The formation of clathrin-coated vesicles occurs continuously in non-dividing cells, but is shut down during mitosis, when clathrin concentrates at the spindle apparatus. Here, we show that clathrin stabilizes fibres of the mitotic spindle to aid congression of chromosomes. Clathrin bound to the spindle directly by the amino-terminal domain of clathrin heavy chain. Depletion of clathrin heavy chain using RNA interference prolonged mitosis; kinetochore fibres were destabilized, leading to defective congression of chromosomes to the metaphase plate and persistent activation of the spindle checkpoint. Normal mitosis was rescued by clathrin triskelia but not the N-terminal domain of clathrin heavy chain, indicating that stabilization of kinetochore fibres was dependent on the unique structure of clathrin. The importance of clathrin for normal mitosis may be relevant to understanding human cancers that involve gene fusions of clathrin heavy chain.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitotic cell cycle involved_inIMP 8553642PMID:15858577UniProt 
receptor-mediated endocytosis involved_inIMP 8553642PMID:15858577UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
spindle located_inIDA 8553642PMID:15858577UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Cltc  (clathrin heavy chain)


Additional Information