RGD Reference Report - Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division. - Rat Genome Database

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Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division.

Authors: Cho, H  Kehrl, JH 
Citation: Cho H and Kehrl JH, J Cell Biol. 2007 Jul 16;178(2):245-55.
RGD ID: 8553256
Pubmed: PMID:17635935   (View Abstract at PubMed)
PMCID: PMC2064444   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200604114   (Journal Full-text)

At the plasma membrane, heterotrimeric G proteins act as molecular switches to relay signals from G protein-coupled receptors; however, G(alpha) subunits also have receptor-independent functions at intracellular sites. Regulator of G protein signaling (RGS) 14, which enhances the intrinsic GTPase activity of G(ialpha) proteins, localizes in centrosomes, which suggests the coexpression of G(ialpha). We show expression of G(ialpha1), G(ialpha2), and G(ialpha3) in the centrosomes and at the midbody. Fluorescence resonance energy transfer analysis confirms a direct interaction between RGS14 and G(ialpha1) in centrosomes. Expression of GTPase-deficient G(ialpha1) results in defective cytokinesis, whereas that of wild-type or GTPase-deficient G(ialpha3) causes prolonged mitosis. Cells treated with pertussis toxin, with reduced expression of G(ialpha1), G(ialpha2), and G(ialpha3) or with decreased expression of RGS14 also exhibit cytokinesis defects. These results suggest that G(ialpha) proteins and their regulators at these sites may play essential roles during mammalian cell division.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
centrosome located_inIDA 8553256PMID:17635935UniProt 
cytoplasm located_inIDA 8553256PMID:17635935UniProt 
plasma membrane located_inIDA 8553256PMID:17635935UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P974928553256PMID:17635935UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Gnai1  (G protein subunit alpha i1)


Additional Information