RGD Reference Report - Cloning and characterization of rat transient receptor potential-melastatin 4 (TRPM4). - Rat Genome Database

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Cloning and characterization of rat transient receptor potential-melastatin 4 (TRPM4).

Authors: Yoo, JC  Yarishkin, OV  Hwang, EM  Kim, E  Kim, DG  Park, N  Hong, SG  Park, JY 
Citation: Yoo JC, etal., Biochem Biophys Res Commun. 2010 Jan 1;391(1):806-11. doi: 10.1016/j.bbrc.2009.11.142. Epub 2009 Nov 27.
RGD ID: 8553249
Pubmed: PMID:19945433   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2009.11.142   (Journal Full-text)

Transient receptor potential-melastatin 4 (TRPM4) is a Ca(2+)-activated, but Ca(2+)-impermeable, cation channel. Increasing [Ca(2+)](i) induce current activation and reduction through TRPM4 channels. Several TRPM4 isoforms are expressed in mice and humans, but rat TRPM4 (rTRPM4) has not been previously identified. Here, we identified, cloned, and characterized two rTRPM4 isoforms, rTRPM4a and rTRPM4b, using 5'-RACE-PCR. rTRPM4b channel activity increased with [Ca(2+)](i) in a dose-dependent manner. However, the rTRPM4b Ca(2+)-dependent activity at negative potentials differed from that of human TRPM4b (hTRPM4b), even though both represent full-length proteins. Additionally, rTRPM4b showed a slightly different single-channel current amplitude and open time distribution than hTRPM4b. However, rTRPM4a, which lacks the N-terminal region of rTRPM4b, and hTRPM4a had no similar functional channel activities. Furthermore, we characterized splicing regions, tissue distribution, and cellular localization of these isoforms. Unlike rTRPM4a, rTRPM4b was localized to the membrane at high levels, suggesting that rTRPM4b is the functionally active channel.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endoplasmic reticulum located_inIDA 8553249PMID:19945433UniProt 
Golgi apparatus located_inIDA 8553249PMID:19945433UniProt 
plasma membrane located_inIDA 8553249PMID:19945433UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium-activated cation channel activity enablesIDA 8553249PMID:19945433UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Trpm4  (transient receptor potential cation channel, subfamily M, member 4)


Additional Information