RGD Reference Report - Modulation of immune signaling, bacterial clearance, and corneal integrity by toll-like receptors during streptococcus pneumoniae keratitis. - Rat Genome Database

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Modulation of immune signaling, bacterial clearance, and corneal integrity by toll-like receptors during streptococcus pneumoniae keratitis.

Authors: Tullos, NA  Thompson, HW  Taylor, SD  Sanders, M  Norcross, EW  Tolo, I  Moore, Q  Marquart, ME 
Citation: Tullos NA, etal., Curr Eye Res. 2013 Oct;38(10):1036-48. doi: 10.3109/02713683.2013.804094. Epub 2013 Jul 10.
RGD ID: 8552886
Pubmed: PMID:23841825   (View Abstract at PubMed)
PMCID: PMC9335516   (View Article at PubMed Central)
DOI: DOI:10.3109/02713683.2013.804094   (Journal Full-text)

PURPOSE: Bacterial keratitis, without effective antimicrobial treatment, leads to poor patient prognosis. Even after bacterial clearance, the host inflammatory response can contribute to corneal damage. Though Streptococcus pneumoniae (pneumococcus) is a common cause of bacterial keratitis, the role of host innate immunity during pneumococcal keratitis is not well characterized. This study investigated the role of Toll-like receptors (TLRs) during pneumococcal keratitis. MATERIALS AND METHODS: C57BL/6, as well as TLR2(-/-) and TLR4(-/-) mice, were infected with S. pneumoniae, and infected corneas were examined for 21 days. Quantitative real-time reverse-transcriptase polymerase chain reaction was performed using primers for genes involved in the inflammatory response and TLR signaling. Bacterial survival and leukocyte invasion were examined over a 72-h period. RESULTS: The corneal expression of TLR2, TLR4, and other inflammatory genes was increased at 72 h post-infection (p.i.) compared to uninfected C57BL/6 scratch controls. TLR2(-/-) mice showed a significant increase in bacterial survival at 24 h p.i. likely due to decreased neutrophil infiltration; however, after Day 5 p.i. observed clinical scores of TLR2(-/-) and C57BL/6 mice were not significantly different. In contrast, permanent corneal damage was observed for TLR4(-/-) mice over 21 days. Initially, both TLR(-/-) mouse strains exhibited lower expression levels in many immune genes, but returned to similar or elevated levels compared to C57BL/6 mice by 72 h p.i. CONCLUSIONS: TLR2 and TLR4 are involved in the response to pneumococcal keratitis and TLR2 may aid in bacterial clearance by recruitment of neutrophils to the cornea, whereas TLR4 may be necessary to modulate the immune response to limit cellular damage.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Bacterial Keratitis  ISOTlr2 (Mus musculus)8552886; 8552886associated with Pneumococcal InfectionsRGD 
Bacterial Keratitis  ISOTlr4 (Mus musculus)8552886; 8552886associated with Pneumococcal InfectionsRGD 
Bacterial Keratitis  IMP 8552886; 8552886associated with Pneumococcal InfectionsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Mus musculus)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Homo sapiens)
TLR2  (toll like receptor 2)
TLR4  (toll like receptor 4)


Additional Information