RGD Reference Report - Higher expression of Toll-like receptors 2, 3, 4, and 8 in ocular Behcet's disease. - Rat Genome Database

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Higher expression of Toll-like receptors 2, 3, 4, and 8 in ocular Behcet's disease.

Authors: Liu, X  Wang, C  Ye, Z  Kijlstra, A  Yang, P 
Citation: Liu X, etal., Invest Ophthalmol Vis Sci. 2013 Sep 5;54(9):6012-7. doi: 10.1167/iovs.13-12159.
RGD ID: 8552883
Pubmed: PMID:23908180   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.13-12159   (Journal Full-text)

PURPOSE: To investigate the role of Toll-like receptors (TLRs) 2, 3, 4, and 8 in the pathogenesis of Behcet's disease (BD). METHODS: Sixteen patients with active ocular BD and 16 healthy volunteers were included in this study. Total RNA was isolated from PBMCs to determine mRNA levels of TLRs, including TLR2, TLR3, TLR4, and TLR8. Cell surface receptor activity of these TLRs was investigated by FACS analysis. Monocytes and naive T cells from patients and controls were cultured with or without TLR ligands, such as LPS, PGN, R848, or PolyI:C. Culture supernatants were collected and IL-17, IL-1beta, and IL-23 were analyzed by ELISA. RESULTS: A markedly higher expression at the mRNA and protein level of TLR2, TLR3, TLR4, and TLR8 was observed in active BD patients as compared with controls. Significantly higher levels of IL-1beta and IL-23 were detected in the supernatants of monocytes stimulated with LPS or PGN. A significantly higher level of IL-17 was observed in the supernatants of naive T cells and monocytes stimulated with LPS or PGN in BD patients as compared with controls. Upon stimulation with R848 or PolyI:C, the levels of IL-17 in the supernatants of naive T cells and monocytes and IL-23 levels in the supernatants of monocytes were not different between BD patients and controls. CONCLUSIONS: A higher expression of TLRs may be involved in the pathogenesis of BD.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Behcet's disease  IEP 8552883; 8552883protein and mRNA:increased expression:peripheral blood mononuclear cellRGD 
Behcet's disease  ISOTLR2 (Homo sapiens)8552883; 8552883protein and mRNA:increased expression:peripheral blood mononuclear cellRGD 
Behcet's disease  ISOTLR3 (Homo sapiens)8552883; 8552883protein and mRNA:increased expression:peripheral blood mononuclear cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Tlr2  (toll-like receptor 2)
Tlr3  (toll-like receptor 3)

Genes (Mus musculus)
Tlr2  (toll-like receptor 2)
Tlr3  (toll-like receptor 3)

Genes (Homo sapiens)
TLR2  (toll like receptor 2)
TLR3  (toll like receptor 3)


Additional Information