RGD Reference Report - Prostaglandin E(2) enhances proliferation, dedifferentiation and stem-like properties of rat retinal Muller glial cells in vitro. - Rat Genome Database

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Prostaglandin E(2) enhances proliferation, dedifferentiation and stem-like properties of rat retinal Muller glial cells in vitro.

Authors: Wang, L  Lang, LL  Wang, Y  Shi, S  Liu, L 
Citation: Wang L, etal., Ophthalmic Res. 2013;49(2):100-7. doi: 10.1159/000345256. Epub 2012 Dec 18.
RGD ID: 8552382
Pubmed: PMID:23257891   (View Abstract at PubMed)
DOI: DOI:10.1159/000345256   (Journal Full-text)

AIMS: To investigate the differences of gene expression in rat retinal Muller glial cells after basic fibroblast growth factor (bFGF) treatment, and to explore the function of prostaglandin E(2) (PGE(2)) in the proliferation and dedifferentiation of rat retinal Muller glial cells. MATERIALS AND METHODS: A gene expression profile in rat retinal Muller glial cells after bFGF treatment, matching untreated cells, was conducted using gene array technology. The candidate gene selection was performed on GenMAPP/MAPPFinder. The functional effects of PGE(2) on the proliferation and dedifferentiation of Muller cells were further investigated. RESULTS: Gene array analysis identified that 298 genes were upregulated and 293 genes were downregulated. The GenMAPP/MAPPFinder results showed that the PG biosynthetic process had the highest correlation (Z score 8.803) with the stem cell characteristics of Muller cells. The quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results showed that PGE(2) can significantly upregulate the expression of cyclin D(1) in Muller cells. Also, the bromodeoxyuridine and flow cytometry results showed that PGE(2) can significantly increase the proportion of Muller cells in S phase. Furthermore, qRT-PCR showed that the expressions of nestin (a marker of neural stem/precursor cells) and Pax6 (a marker of retinal stem/precursor cells) were significantly upregulated by PGE(2) stimulation, and similar results were obtained by immunofluorescence staining. CONCLUSION: PGE(2) may enhance the proliferation, dedifferentiation, and stem-like properties of rat retinal Muller glial cells in vitro.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to prostaglandin E stimulus  IEP 8552382 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pax6  (paired box 6)


Additional Information