RGD Reference Report - Effect of electrical stimulation on IGF-1 transcription by L-type calcium channels in cultured retinal Muller cells. - Rat Genome Database

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Effect of electrical stimulation on IGF-1 transcription by L-type calcium channels in cultured retinal Muller cells.

Authors: Sato, T  Fujikado, T  Morimoto, T  Matsushita, K  Harada, T  Tano, Y 
Citation: Sato T, etal., Jpn J Ophthalmol. 2008 May-Jun;52(3):217-23. doi: 10.1007/s10384-008-0533-y. Epub 2008 Jul 27.
RGD ID: 8548847
Pubmed: PMID:18661273   (View Abstract at PubMed)
DOI: DOI:10.1007/s10384-008-0533-y   (Journal Full-text)

PURPOSE: To investigate the effect of electrical stimulation (ES) on the induction of insulin-like growth factor 1 (IGF-1) in cultured retinal Muller cells. METHODS: Muller cells were isolated from rat retinas. ES was applied to Muller cells of passage 1 with biphasic pulses (duration, 1 ms; frequency, 20 Hz; current, 0-10 mA) for 30 min. The mRNA level of IGF-1 was determined by reverse transcription-polymerase chain reaction (RT-PCR) immediately to 2 h after ES. The change of intracellular calcium concentration ([Ca(2+)]) induced by ES was monitored by Ca(2+) imaging with Fura 2-AM. Ca(2+) imaging and RT-PCR were performed with and without the application of l muM nifedipine, an L-type calcium channel blocker. RESULTS: The mRNA level of IGF-1 was increased significantly (P<0.05) by about 1.3-fold immediately after 10 mA ES. [Ca(2+)] began to increase immediately after the start of ES, reached a maximum of approximately 1.8-fold, and continued to increase until about 20 min after the ES. The inductions of IGF-1 transcription and Ca(2+) influx were suppressed by nifedipine. CONCLUSIONS: These results indicate that the enhancement of IGF-1 transcription by ES in cultured Muller cells depends largely on Ca(2+) influx through L-type Ca(2+) channels.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to electrical stimulus  IEP 8548847 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Igf1  (insulin-like growth factor 1)


Additional Information