RGD Reference Report - Investigation of environmental factors for diagnosing malignant potential in oral epithelial dysplasia. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Investigation of environmental factors for diagnosing malignant potential in oral epithelial dysplasia.

Authors: Okazaki, Y  Tanaka, Y  Tonogi, M  Yamane, G 
Citation: Okazaki Y, etal., Oral Oncol. 2002 Sep;38(6):562-73.
RGD ID: 8547871
Pubmed: PMID:12167434   (View Abstract at PubMed)

A study was conducted in rats with early tongue carcinoma induced by 4-nitroquinoline 1-oxide (4NQO), in order to investigate the early diagnosis of malignant potential of epithelial dysplasia. The rat tongue lesions were classified by their severity into three groups corresponding to early cancer, dysplasia and no change. The grade of epithelial changes was determined according to 13 items of WHO Epithelial Dysplasia Criteria. The expression levels of p53 and Bcl-2 proteins were detected immunohistochemically, and apoptotic cells were detected using the TUNEL method. In addition, a p53 mutation by lesions was detected. The expression ratio of p53 protein was high in dysplasia, and the ratio of Bcl-2 protein was high in early cancer and dysplasia. The TUNEL-positive cells were observed primarily in the granular layers of the no change cells, and their numbers decreased as the cells shifted to the early cancer stage. The p53 mutation was detected using a microdissection method in dysplasia, where it was found in three out of nine lesions. All the mutations in dysplasia detected were on the same codon that was found to be mutated in the early cancer. These results indicate that the association between the p53 mutation and histological changes in carcinogenesis epithelial dysplasia is strong, and that both the identification of p53- and Bcl-2-positive epithelium, and decrease in the TUNEL positive ratio, were useful for the diagnosis of the malignant potential of precancerous lesions.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
oral mucosa leukoplakia  ISOBcl2 (Rattus norvegicus)8547871; 8547871protein:increased expression:tongue:RGD 
oral mucosa leukoplakia  IEP 8547871; 8547871protein:increased expression:tongue:RGD 
oral mucosa leukoplakia  ISOTp53 (Rattus norvegicus)8547871; 8547871protein:increased expression:tongue:RGD 
oral mucosa leukoplakia  IMP 8547871DNA:mutations:cds:RGD 
Tongue Neoplasms  ISOBcl2 (Rattus norvegicus)8547871; 8547871protein:increased expression:tongue:RGD 
Tongue Neoplasms  IEP 8547871protein:increased expression:tongue:RGD 
Tongue Neoplasms  ISOTp53 (Rattus norvegicus)8547871; 8547871DNA:mutations:cds:RGD 
Tongue Neoplasms  IMP 8547871DNA:mutations:cds:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bcl2  (BCL2, apoptosis regulator)
Tp53  (tumor protein p53)

Genes (Mus musculus)
Bcl2  (B cell leukemia/lymphoma 2)
Trp53  (transformation related protein 53)

Genes (Homo sapiens)
BCL2  (BCL2 apoptosis regulator)
TP53  (tumor protein p53)


Additional Information