RGD Reference Report - Immunoaffinity purification of active rat recombinant 1,25-dihydroxyvitamin D3 receptor. - Rat Genome Database

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Immunoaffinity purification of active rat recombinant 1,25-dihydroxyvitamin D3 receptor.

Authors: Li, Z  Prahl, JM  Hellwig, W  DeLuca, HF 
Citation: Li Z, etal., Arch Biochem Biophys. 1994 May 1;310(2):347-51.
RGD ID: 8158087
Pubmed: PMID:8179318   (View Abstract at PubMed)

We have developed a large-scale immunoaffinity purification procedure for the recombinant vitamin D receptor. The purified receptor is homogeneous, and is bound by 1,25-dihydroxyvitamin D3 with a Kd of 5 x 10(-10) M. The isolated receptor binds to the osteocalcin vitamin D response element in the presence of porcine intestinal nuclear extract stripped of endogenous vitamin D receptor as well. However, the binding of D3 and the vitamin D3 response element does not completely assure a native conformation of the protein. The availability of large quantities of highly purified active vitamin D receptor makes possible detailed structural analysis.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcitriol binding  IDA 8158087 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Vdr  (vitamin D receptor)


Additional Information