RGD Reference Report - Dry eye-induced conjunctival epithelial squamous metaplasia is modulated by interferon-gamma. - Rat Genome Database

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Dry eye-induced conjunctival epithelial squamous metaplasia is modulated by interferon-gamma.

Authors: De Paiva, CS  Villarreal, AL  Corrales, RM  Rahman, HT  Chang, VY  Farley, WJ  Stern, ME  Niederkorn, JY  Li, DQ  Pflugfelder, SC 
Citation: De Paiva CS, etal., Invest Ophthalmol Vis Sci. 2007 Jun;48(6):2553-60.
RGD ID: 8157601
Pubmed: PMID:17525184   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.07-0069   (Journal Full-text)

PURPOSE: To investigate the role of interferon (IFN)-gamma in the pathogenesis of conjunctival squamous metaplasia in dry eye. METHODS: Experimental dry eye was created by subjecting C57BL/6 and IFN-gamma-knockout mice to desiccating environmental stress for 5 or 10 days. T-cell antigens and IFN-gamma were detected by immunohistochemistry. Goblet cells were counted in periodic acid Schiff (PAS)-stained sections. Expression of small, proline-rich protein (SPRR)-2 was evaluated by confocal microscopy. Tear IFN-gamma was measured by immunobead assay. RESULTS: Dry eye promoted migration of CD4+ T cells and IFN-gamma+ cells into goblet cell zones of the conjunctiva and increased the concentration of IFN-gamma in tears. This migration was accompanied by progressive goblet cell loss and an increase in SPRR-2 expression in the conjunctival epithelium. A significant inverse correlation was observed between the density of infiltrating CD4+ T cells and goblet cells. Dry eye had no effect on conjunctival goblet cell density in IFN-gamma-knockout mice; however, exogenous administration of IFN-gamma significantly decreased goblet cell density after 5 days. CONCLUSIONS: Conjunctival epithelial response to experimental dryness is related to the degree of CD4+ T-cell infiltration and the level of IFN-gamma production. These findings suggest that IFN-gamma plays a pivotal role in promoting conjunctival squamous metaplasia in dry eye, and they provide insight into the immune pathogenesis of keratoconjunctivitis sicca.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dry eye syndrome  ISOIfng (Mus musculus)8157601; 8157601protein:increased expression:tear:RGD 
dry eye syndrome  IDA 8157601 RGD 
dry eye syndrome  IEP 8157601protein:increased expression:tear:RGD 
dry eye syndrome  IMP 8157601 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ifng  (interferon gamma)

Genes (Mus musculus)
Ifng  (interferon gamma)

Genes (Homo sapiens)
IFNG  (interferon gamma)


Additional Information