RGD Reference Report - Increased expression of IRE1alpha and stress-related signal transduction proteins in ischemia-reperfusion injured retina. - Rat Genome Database

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Increased expression of IRE1alpha and stress-related signal transduction proteins in ischemia-reperfusion injured retina.

Authors: Hata, N  Oshitari, T  Yokoyama, A  Mitamura, Y  Yamamoto, S 
Citation: Hata N, etal., Clin Ophthalmol. 2008 Dec;2(4):743-52.
RGD ID: 7495824
Pubmed: PMID:19668425   (View Abstract at PubMed)
PMCID: PMC2699777   (View Article at PubMed Central)

The purpose of this study was to determine whether the expression of ER stress-related factors IRE1alpha, apoptosis signal-regulating kinase 1 (ASK1), SAPK/ERK kinase 1 (SEK1) and c-Jun N-terminal kinase (JNK) is associated with the damaged retinal neurons induced by ischemia-reperfusion injury. After 60 minutes of ischemia, the rat retinas were reperfused, and retinas were isolated and fixed after 6, 9, 12, 18, and 24 hours, and 2, 5, and 9 days of reperfusion. Cryosections were immunostained with Fluoro-Jade B, a degenerating neuron marker to label degenerating neurons. Semi-quantitative analysis of the expression of IRE1alpha, ASK1, SEK1, and JNK were performed in both control and ischemic retinas. In ischemic retinas, the intensities of IRE1alpha immunoreactivity in the ganglion cell layer (GCL) were significantly higher than in the control retinas. In ischemic retinas, the numbers of SEK1-, ASK1-, and JNK-positive cells were significantly increased in the GCL compared to those in the control retinas. In addition, the cells that were positive for SEK1-, ASK1-, and JNK were also positive for Fluoro-Jade B-positive cells. These results indicate that the increased expression of ER stress-related factors was, in part, associated with the retinal neuronal abnormalities after ischemia-reperfusion injury in rat retinas.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Retina Reperfusion Injury  ISOMap2k4 (Rattus norvegicus)7495824; 7495824 RGD 
Retina Reperfusion Injury  IEP 7495824 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Map2k4  (mitogen activated protein kinase kinase 4)

Genes (Mus musculus)
Map2k4  (mitogen-activated protein kinase kinase 4)

Genes (Homo sapiens)
MAP2K4  (mitogen-activated protein kinase kinase 4)


Additional Information