RGD Reference Report - Detection of GST1 gene deletion by the polymerase chain reaction and its possible correlation with stomach cancer in Japanese. - Rat Genome Database

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Detection of GST1 gene deletion by the polymerase chain reaction and its possible correlation with stomach cancer in Japanese.

Authors: Harada, S  Misawa, S  Nakamura, T  Tanaka, N  Ueno, E  Nozoe, M 
Citation: Harada S, etal., Hum Genet. 1992 Sep-Oct;90(1-2):62-4.
RGD ID: 7488951
Pubmed: PMID:1427788   (View Abstract at PubMed)

A homozygous gene deletion at the GST1 locus of genomic DNA isolated from peripheral blood was investigated for its relationship with several types of cancer using the polymerase chain reaction (PCR) technique. DNA samples were prepared from blood obtained from 128 healthy blood donors and 150 patients with cancer or chronic hepatitis. PCR primers were prepared based on the human cDNA sequence and the intron/exon sequences of the rat Yb2 gene. The amplified sequence between exons 5 and 6 including intron 5 showed very clearly the presence of absence of the GST1 gene, after electrophoresis in a 2% agarose gel. Segregation of the presence and absence of PCR product from samples of twins and their parents indicated that presence involves homozygous or heterozygous normal GST1 genotypes while absence involves only homozygous gene deletion. The patients with stomach cancer had a significantly higher frequency of gene deletion than did the healthy controls (P < 0.005). Thus, GST1 deletion may be a possible genetic marker for early detection of a group at high risk of stomach cancer.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
stomach cancer susceptibilityIAGP 7488951DNA:deletion:cds (human)RGD 
stomach cancer susceptibilityISOGSTM1 (Homo sapiens)7488951; 7488951DNA:deletion:cds (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gstm1  (glutathione S-transferase mu 1)

Genes (Mus musculus)
Gstm1  (glutathione S-transferase, mu 1)

Genes (Homo sapiens)
GSTM1  (glutathione S-transferase mu 1)

Objects referenced in this article
Gene Gstm2 glutathione S-transferase, mu 2 Mus musculus
Gene Gstm2 glutathione S-transferase mu 2 Rattus norvegicus

Additional Information