RGD Reference Report - Identification of p115 as a PLCgamma1-binding protein and the role of Src homology domains of PLCgamma1 in the vesicular transport. - Rat Genome Database

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Identification of p115 as a PLCgamma1-binding protein and the role of Src homology domains of PLCgamma1 in the vesicular transport.

Authors: Han, SJ  Lee, JH  Kim, CG  Hong, SH 
Citation: Han SJ, etal., Biochem Biophys Res Commun 2003 Jan 17;300(3):649-55.
RGD ID: 734467
Pubmed: PMID:12507498   (View Abstract at PubMed)

In order to gain further insight into the function(s) of PLCgamma1, we tried to identify the binding partners that can interact with the SH223 domains of PLCgamma1. Immunoscreening was performed with the purified antisera that are specific to SH223-binding proteins. Several immunoreactive clones were identified as the putative binding proteins and one of them was identified as p115. p115 was reported to be required for transcytotic fusion and subsequent binding of the vesicles to the target membrane. The interaction between PLCgamma1 and p115 was specific to carboxyl-terminal SH2 domain and SH3 domain of PLCgamma1, and also confirmed by biochemical approaches such as co-immunoprecipitation, pull-down assay, and glycerol gradient fractionation. To further characterize the role of SH domains of PLCgamma1 in the vesicle transport pathway, secreted form of alkaline phosphatase (SEAP) reporter assay was carried out. When the SH2 and/or SH3 domains of PLCgamma1 were deleted, the secretion of SEAP was significantly reduced. These findings indicate that the SH2 and SH3 domains of PLCgamma1 may play a role(s) in the process of the vesicle transport via interaction with other vesicle-associated proteins such as p115.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIUso1 (Rattus norvegicus)734467 RGD 
protein binding  IPIPlcg1 (Rattus norvegicus)734467 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Plcg1  (phospholipase C, gamma 1)
Uso1  (USO1 vesicle transport factor)


Additional Information