RGD Reference Report - Combined insulin treatment and intense exercise training improved basal cardiac function and Ca(2+)-cycling proteins expression in type 1 diabetic rats. - Rat Genome Database

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Combined insulin treatment and intense exercise training improved basal cardiac function and Ca(2+)-cycling proteins expression in type 1 diabetic rats.

Authors: Le Douairon Lahaye, S  Gratas-Delamarche, A  Malarde, L  Zguira, S  Vincent, S  Lemoine Morel, S  Carre, F  Rannou Bekono, F 
Citation: Le Douairon Lahaye S, etal., Appl Physiol Nutr Metab. 2012 Feb;37(1):53-62. doi: 10.1139/h11-127. Epub 2011 Dec 20.
RGD ID: 7327207
Pubmed: PMID:22185592   (View Abstract at PubMed)
DOI: DOI:10.1139/h11-127   (Journal Full-text)

This study investigated the effects of 8 weeks of intense exercise training combined with insulin treatment on the Ca(2+)-cycling protein complex expression and their functional consequences on cardiac function in type 1 diabetic rat hearts. Diabetic Wistar rats were randomly assigned into the following groups: received no treatment, insulin-treated diabetic, trained diabetic, and trained insulin-treated diabetic. A control group was also included. Insulin treatment and (or) treadmill intense exercise training were conducted over 8 weeks. Basal cardiac function was evaluated by Langendorff technique. Cardiac expression of the main Ca(2+)-cycling proteins (RyR2, FKBP 12.6, SERCA2, PLB, NCX1) was assessed by Western blot. Diabetes altered basal cardiac function (+/-dP/dt) and decrease the expression of the main Ca(2+)-cycling proteins expression: RyR2, SERCA2, and NCX1 (p < 0.05). Whereas combined treatment was not able to normalize -dP/dt, it succeeded to normalize +dP/dt of diabetic rats (p < 0.05). Moreover, both insulin and intense exercise training, applied solely, increased the expression of the Ca(2+)-cycling proteins: RyR2, SERCA2, PLB. and NCX1 (p < 0.05). But this effect was higher when the 2 treatments were combined. These data are the first to show that combined insulin treatment and intense exercise training during diabetes synergistically act on the expression of the main Ca(2+)-cycling proteins, providing insights into mechanisms by which the dual treatment during diabetes improves cardiac function.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Diabetic Cardiomyopathies  ISOPln (Rattus norvegicus)7327207; 7327207associated with Diabetes Mellitus more ...RGD 
Diabetic Cardiomyopathies  IEP 7327207associated with Diabetes Mellitus more ...RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to insulin  IEP 7327207 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pln  (phospholamban)

Genes (Mus musculus)
Pln  (phospholamban)

Genes (Homo sapiens)
PLN  (phospholamban)


Additional Information