RGD Reference Report - Luteolin inhibits apoptosis and improves cardiomyocyte contractile function through the PI3K/Akt pathway in simulated ischemia/reperfusion. - Rat Genome Database

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Luteolin inhibits apoptosis and improves cardiomyocyte contractile function through the PI3K/Akt pathway in simulated ischemia/reperfusion.

Authors: Fang, F  Li, D  Pan, H  Chen, D  Qi, L  Zhang, R  Sun, H 
Citation: Fang F, etal., Pharmacology. 2011;88(3-4):149-58. doi: 10.1159/000330068. Epub 2011 Sep 16.
RGD ID: 7327186
Pubmed: PMID:21934351   (View Abstract at PubMed)
DOI: DOI:10.1159/000330068   (Journal Full-text)

Luteolin, a naturally occurring polyphenol flavonoid, has demonstrated to exert myocardial protection effects. However, the mechanisms have not been fully elucidated. In the present study, we investigated whether luteolin pretreatment was associated with cardioprotection in a rat ischemia/reperfusion (I/R) model. Luteolin significantly not only restored contractility of the left ventricle, but also reduced the infarct size and lactate dehydrogenase leakage during I/R. In addition, luteolin pretreatment significantly improved cardiomyocyte shortening amplitude, decreased the apoptotic rate, upregulated Bcl-2 expression, downregulated Bax expression and raised the Bcl-2/Bax ratio under a simulated ischemia/reperfusion (SI/R) condition. Moreover, luteolin pretreatment increased protein kinase B (Akt) phosphorylation, phospholamban phosphorylation and the expression of sarcoplasmic reticulum calcium ATPase following SI/R. The phosphoinositide 3-kinase (PI3K)/Akt pathway is one of the most important intracellular survival signal pathways. To determine whether luteolin-induced cardioprotection was mediated by the PI3K/Akt pathway, we utilized the PI3K inhibitor LY294002. Inhibition of Akt activity markedly abolished luteolin-induced positive contraction and inhibition of apoptosis in SI/R cardiomyocytes. These results showed that luteolin inhibits apoptosis and improves cardiomyocyte contractile function at least partly through the PI3K/Akt pathway in SI/R.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Reperfusion Injury treatmentISOPln (Rattus norvegicus)7327186; 7327186 RGD 
Myocardial Reperfusion Injury treatmentIDA 7327186 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pln  (phospholamban)

Genes (Mus musculus)
Pln  (phospholamban)

Genes (Homo sapiens)
PLN  (phospholamban)


Additional Information