RGD Reference Report - GEBR-7b, a novel PDE4D selective inhibitor that improves memory in rodents at non-emetic doses. - Rat Genome Database

RGD Reference Report - GEBR-7b, a novel PDE4D selective inhibitor that improves memory in rodents at non-emetic doses. - Rat Genome Database

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GEBR-7b, a novel PDE4D selective inhibitor that improves memory in rodents at non-emetic doses.
Authors:	Bruno, O Fedele, E Prickaerts, J Parker, LA Canepa, E Brullo, C Cavallero, A Gardella, E Balbi, A Domenicotti, C Bollen, E Gijselaers, HJ Vanmierlo, T Erb, K Limebeer, CL Argellati, F Marinari, UM Pronzato, MA Ricciarelli, R
Citation:	Bruno O, etal., Br J Pharmacol. 2011 Dec;164(8):2054-63. doi: 10.1111/j.1476-5381.2011.01524.x.
RGD ID:	7327158
Pubmed:	PMID:21649644 (View Abstract at PubMed)
PMCID:	PMC3246667 (View Article at PubMed Central)
DOI:	DOI:10.1111/j.1476-5381.2011.01524.x (Journal Full-text)
BACKGROUND AND PURPOSE: Strategies designed to enhance cerebral cAMP have been proposed as symptomatic treatments to counteract cognitive deficits. However, pharmacological therapies aimed at reducing PDE4, the main class of cAMP catabolizing enzymes in the brain, produce severe emetic side effects. We have recently synthesized a 3-cyclopentyloxy-4-methoxybenzaldehyde derivative, structurally related to rolipram, and endowed with selective PDE4D inhibitory activity. The aim of the present study was to investigate the effect of the new drug, namely GEBR-7b, on memory performance, nausea, hippocampal cAMP and amyloid-beta (Abeta) levels. EXPERIMENTAL APPROACH: To measure memory performance, we performed object recognition tests on rats and mice treated with GEBR-7b or rolipram. The emetic potential of the drug, again compared with rolipram, was evaluated in rats using the taste reactivity test and in mice using the xylazine/ketamine anaesthesia test. Extracellular hippocampal cAMP was evaluated by intracerebral microdialysis in freely moving rats. Levels of soluble Abeta peptides were measured in hippocampal tissues and cultured N2a cells by elisa. KEY RESULTS: GEBR-7b increased hippocampal cAMP, did not influence Abeta levels and improved spatial, as well as object memory performance in the object recognition tests. The effect of GEBR-7b on memory was 3 to 10 times more potent than that of rolipram, and its effective doses had no effect on surrogate measures of emesis in rodents. CONCLUSION AND IMPLICATIONS: Our results demonstrate that GEBR-7b enhances memory functions at doses that do not cause emesis-like behaviour in rodents, thus offering a promising pharmacological perspective for the treatment of memory impairment.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
memory		IMP		7327158		RGD

Objects Annotated

Genes (Rattus norvegicus)

Pde4d (phosphodiesterase 4D)

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