RGD Reference Report - Phosphodiesterase isoform-specific expression induced by traumatic brain injury. - Rat Genome Database

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Phosphodiesterase isoform-specific expression induced by traumatic brain injury.

Authors: Oliva AA, JR  Kang, Y  Furones, C  Alonso, OF  Bruno, O  Dietrich, WD  Atkins, CM 
Citation: Oliva AA Jr, etal., J Neurochem. 2012 Dec;123(6):1019-29. doi: 10.1111/jnc.12049. Epub 2012 Nov 1.
RGD ID: 7327147
Pubmed: PMID:23057870   (View Abstract at PubMed)
PMCID: PMC3514616   (View Article at PubMed Central)
DOI: DOI:10.1111/jnc.12049   (Journal Full-text)

Traumatic brain injury (TBI) results in significant inflammation which contributes to the evolving pathology. Previously, we have demonstrated that cyclic AMP (cAMP), a molecule involved in inflammation, is down-regulated after TBI. To determine the mechanism by which cAMP is down-regulated after TBI, we determined whether TBI induces changes in phosphodiesterase (PDE) expression. Adult male Sprague Dawley rats received moderate parasagittal fluid-percussion brain injury (FPI) or sham injury, and the ipsilateral, parietal cortex was analyzed by western blotting. In the ipsilateral parietal cortex, expression of PDE1A, PDE4B2, and PDE4D2, significantly increased from 30 min to 24 h post-injury. PDE10A significantly increased at 6 and 24 h after TBI. Phosphorylation of PDE4A significantly increased from 6 h to 7 days post-injury. In contrast, PDE1B, PD4A5, and PDE4A8 significantly decreased after TBI. No changes were observed with PDE1C, PDE3A, PDE4B1/3, PDE4B4, PDE4D3, PDE4D4, PDE8A, or PDE8B. Co-localization studies showed that PDE1A, PDE4B2, and phospho-PDE4A were neuronally expressed, whereas PDE4D2 was expressed in neither neurons nor glia. These findings suggest that therapies to reduce inflammation after TBI could be facilitated with targeted therapies, in particular for PDE1A, PDE4B2, PDE4D2, or PDE10A.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Brain Injuries  ISOPde4d (Rattus norvegicus)7327147; 7327147mRNA more ...RGD 
Brain Injuries  IEP 7327147mRNA more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pde4d  (phosphodiesterase 4D)

Genes (Mus musculus)
Pde4d  (phosphodiesterase 4D, cAMP specific)

Genes (Homo sapiens)
PDE4D  (phosphodiesterase 4D)


Additional Information