RGD Reference Report - Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation. - Rat Genome Database

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Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation.

Authors: Andersson, S  Bishop, RW  Russell, DW 
Citation: Andersson S, etal., J Biol Chem 1989 Sep 25;264(27):16249-55.
RGD ID: 730219
Pubmed: PMID:2476440   (View Abstract at PubMed)
PMCID: PMC4448950   (View Article at PubMed Central)

The conversion of testosterone into the more potent androgen, dihydrotestosterone, catalyzed by the enzyme steroid 5 alpha-reductase, is required for the differentiation of male external genitalia. Here, we report the isolation of cDNA clones encoding the rat steroid 5 alpha-reductase using expression cloning in Xenopus oocytes. DNA sequence analysis demonstrates that the liver and ventral prostate forms of steroid 5 alpha-reductases are identical hydrophobic proteins of 29 kDa. The amount of steroid 5 alpha-reductase mRNA in liver increased in response to castration, but remained unchanged in the prostate. Testosterone administration to castrates induced expression of mRNA in the prostate but had no effect on liver. The data suggest that the steroid 5 alpha-reductase gene is differentially regulated by testosterone in androgen-responsive versus non-responsive tissues.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
male sex differentiation  TAS 730219 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
3-oxo-5-alpha-steroid 4-dehydrogenase activity  TAS 730219 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Srd5a1  (steroid 5 alpha-reductase 1)


Additional Information