RGD Reference Report - SNAREs in native plasma membranes are active and readily form core complexes with endogenous and exogenous SNAREs. - Rat Genome Database

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SNAREs in native plasma membranes are active and readily form core complexes with endogenous and exogenous SNAREs.

Authors: Lang, T  Margittai, M  Holzler, H  Jahn, R 
Citation: Lang T, etal., J Cell Biol 2002 Aug 19;158(4):751-60.
RGD ID: 730202
Pubmed: PMID:12177041   (View Abstract at PubMed)
PMCID: PMC2174015   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200203088   (Journal Full-text)

During neuronal exocytosis, the vesicle-bound soluble NSF attachment protein (SNAP) receptor (SNARE) synaptobrevin 2 forms complexes with the plasma membrane-bound SNAREs syntaxin 1A and SNAP25 to initiate the fusion reaction. However, it is not known whether in the native membrane SNAREs are constitutively active or whether they are unable to enter SNARE complexes unless activated before membrane fusion. Here we used binding of labeled recombinant SNAREs to inside-out carrier supported plasma membrane sheets of PC12 cells to probe for the activity of endogenous SNAREs. Binding was specific, saturable, and depended on the presence of membrane-resident SNARE partners. Our data show that virtually all of the endogenous syntaxin 1 and SNAP-25 are highly reactive and readily form SNARE complexes with exogenously added SNAREs. Furthermore, complexes between endogenous SNAREs were not detectable when the membranes are freshly prepared, but they slowly form upon prolonged incubation in vitro. We conclude that the activity of membrane-resident SNAREs is not downregulated by control proteins but is constitutively active even if not engaged in fusion events.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
exocytosis  TAS 730202 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
SNAP receptor activity  TAS 730202 RGD 
SNARE binding  IDA 730202 RGD 
syntaxin-1 binding  IDA 730202 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Snap25  (synaptosome associated protein 25)

Objects referenced in this article
Gene Stx1a syntaxin 1A Rattus norvegicus
Gene Vamp2 vesicle-associated membrane protein 2 Rattus norvegicus

Additional Information