RGD Reference Report - The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its N-terminal domain. - Rat Genome Database

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The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its N-terminal domain.

Authors: Suzuki, T  Fujikura, K  Koyama, H  Matsuzaki, T  Takahashi, Y  Takata, K 
Citation: Suzuki T, etal., Eur J Cell Biol 2001 Dec;80(12):765-74.
RGD ID: 730194
Pubmed: PMID:11831390   (View Abstract at PubMed)
DOI: DOI:10.1078/0171-9335-00204   (Journal Full-text)

SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of SGLT1, we constructed an N-terminal deletion clone series of rat SGLT1 and analyzed the localization of the respective products in Madin-Darby canine kidney (MDCK) cells. The products of N-terminal deletion clones up to the 19th amino acid were localized at the apical plasma membrane, whereas the products of N-terminal 20- and 23-amino-acid deletion clones were localized along the entire plasma membrane. Since single-amino-acid mutations of either D28N or D28G in the N-terminal domain give rise to glucose/galactose malabsorption disease, we examined the localization of these mutants. The products of D28N and D28G clones were localized in the cytoplasm, showing that the aspartic acid-28 may be essential for the delivery of SGLT1 to the plasma membrane. These results suggest that a short amino acid sequence of the N-terminal domain of SGLT1 plays important roles in plasma membrane targeting and specific apical localization of the protein.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glucose transmembrane transport  TAS 730194 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane  IDA 730194 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glucose:sodium symporter activity  TAS 730194 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc5a1  (solute carrier family 5 member 1)


Additional Information