RGD Reference Report - Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain. - Rat Genome Database

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Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Authors: Vician, L  Lim, IK  Ferguson, G  Tocco, G  Baudry, M  Herschman, HR 
Citation: Vician L, etal., Proc Natl Acad Sci U S A 1995 Mar 14;92(6):2164-8.
RGD ID: 730180
Pubmed: PMID:7892240   (View Abstract at PubMed)
PMCID: PMC42444   (View Article at PubMed Central)

Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought to play a role in depolarization-induced, calcium-mediated exocytosis and neurotransmitter release. SytIV mRNA accumulation is transiently induced in PC12 cells by potassium depolarization, calcium ionophore, ATP, and forskolin. In contrast, growth factors and phorbol 12-myristate 13-acetate induce little or no SytIV mRNA accumulation. Kainic acid-induced seizures in rats are followed by accumulation of SytIV message in the hippocampus and piriform cortex. The SytIV gene may provide a direct link between depolarization-induced neuronal gene expression and subsequent modulation of synaptic structure and function.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium-ion regulated exocytosis  NAS 730180 RGD 
neurotransmitter secretion  NAS 730180 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
membrane located_inNAS 730180PMID:7892240ParkinsonsUK-UCL 
synaptic vesicle  NAS 730180 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Syt4  (synaptotagmin 4)


Additional Information