RGD Reference Report - Molecular cloning and characterization of high-affinity carnitine transporter from rat intestine. - Rat Genome Database

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Molecular cloning and characterization of high-affinity carnitine transporter from rat intestine.

Authors: Sekine, T  Kusuhara, H  Utsunomiya-Tate, N  Tsuda, M  Sugiyama, Y  Kanai, Y  Endou, H 
Citation: Sekine T, etal., Biochem Biophys Res Commun 1998 Oct 20;251(2):586-91.
RGD ID: 730132
Pubmed: PMID:9792817   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1998.9521   (Journal Full-text)

Carnitine is an essential component for mitochondrial beta-oxidation of fatty acid. Using the degenerate primers designed for organic anion transporters and an organic cation transporter, we isolated a novel cDNA encoding a carnitine transporter (CT1) from rat intestine. CT1 encodes a 557-amino-acid protein with 12 putative membrane-spanning domains. When expressed in Xenopus oocytes, CT1 mediated a high-affinity transport of L-carnitine (Km = 25 microM). The replacement of extracellular sodium with Li reduced CT1-mediated L-carnitine uptake to 19.8%. CT1 did not transport typical substrates for either organic anion or organic cation transporters, such as p-aminohippurate and tetraethylammonium. Octanoylcarnitine, acetylcarnitine, and gamma-butyrobetaine showed potent inhibitory effects on CT1-mediated L-carnitine uptake; betaine and d-carnitine showed moderate inhibition. CT1 mRNA was strongly expressed in the testis, colon, kidney, and liver and weakly in the skeletal muscle, placenta, small intestine, and brain. No CT1 expression was detected in the heart, spleen, or lung. The present study provides the molecular basis of carnitine transport in the body.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
carnitine transport  IDA 730132 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
carnitine transmembrane transporter activity  IDA 730132 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc22a5  (solute carrier family 22 member 5)


Additional Information