RGD Reference Report - Trefoil peptide expression and goblet cell number in rat intestine: effects of KGF and fasting-refeeding. - Rat Genome Database

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Trefoil peptide expression and goblet cell number in rat intestine: effects of KGF and fasting-refeeding.

Authors: Fernandez-Estivariz, C  Gu, LH  Gu, L  Jonas, CR  Wallace, TM  Pascal, RR  Devaney, KL  Farrell, CL  Jones, DP  Podolsky, DK  Ziegler, TR 
Citation: Fernandez-Estivariz C, etal., Am J Physiol Regul Integr Comp Physiol 2003 Feb;284(2):R564-73.
RGD ID: 730068
Pubmed: PMID:12388439   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpregu.00428.2002   (Journal Full-text)

The trefoil factor family peptides TFF1, TFF2, and TFF3 are important for gut mucosal protection and restitution. Keratinocyte growth factor (KGF) stimulates proliferation and differentiation of epithelial cells with potent effects on goblet cells. To investigate interactions between food intake and KGF, rats were fed ad libitum (control), fasted for 72 h, or fasted for 72 h and then refed for 72 h with or without KGF (3 mg. kg(-1). day(-1)). With fasting, goblet cell number in duodenum increased, TFF3 mRNA in duodenum and jejunum decreased, and TFF3 protein did not change or increased. KGF during fasting stimulated colonic growth, normalized TFF3 mRNA in duodenum and jejunum, and broadly upregulated gut goblet cell number and TFF3 protein expression. With fasting-refeeding, KGF increased small bowel and colonic mucosal growth, goblet cell number, and TFF3 protein but had variable effects on TFF3 mRNA. KGF induced TFF2 mRNA and protein in duodenum and jejunum with both nutritional regimens. We conclude that nutrient availability modifies rat intestinal goblet cell number, TFF3 mRNA, and the gut-trophic effects of KGF in a region-specific manner. KGF enhances TFF2 expression in proximal small bowel and increases goblet cell number and TFF3 protein content throughout the intestine independent of food intake.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
structural molecule activity  NAS 730068 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tff2  (trefoil factor 2)

Objects referenced in this article
Gene Nkx2-1 NK2 homeobox 1 Rattus norvegicus
Gene Tff3 trefoil factor 3 Rattus norvegicus

Additional Information