RGD Reference Report - Cloning of two isoforms of the rat brain Na(+)-Ca2+ exchanger gene and their functional expression in HeLa cells. - Rat Genome Database

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Cloning of two isoforms of the rat brain Na(+)-Ca2+ exchanger gene and their functional expression in HeLa cells.

Authors: Furman, I  Cook, O  Kasir, J  Rahamimoff, H 
Citation: Furman I, etal., FEBS Lett 1993 Mar 15;319(1-2):105-9.
RGD ID: 730019
Pubmed: PMID:8454039   (View Abstract at PubMed)

Two functional isoforms of the rat brain Na(+)-Ca2+ exchanger were isolated from a lambda ZAP hippocampus cDNA library. The open reading frame of clone RBE-1 codes for a protein 935 amino acids long, and that of clone RBE-2 codes for a protein 958 amino acids long. Expression HeLa cells of Na+ gradient dependent Ca2+ transport activity was determined following transfection of the cells with either RBE-1 or RBE-2. Both clones expressed proteins that exchange Na+ with Ca2+ in an electrogenic manner and none of them exhibited a dependency of the antiport on K, since they transported Ca2+ in an Na+ gradient dependent manner in external choline chloride as well.

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Gene Slc8a1 solute carrier family 8 member A1 Rattus norvegicus

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