RGD Reference Report - Comparative analysis of the ontogeny of a sodium-dependent bile acid transporter in rat kidney and ileum. - Rat Genome Database

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Comparative analysis of the ontogeny of a sodium-dependent bile acid transporter in rat kidney and ileum.

Authors: Christie, DM  Dawson, PA  Thevananther, S  Shneider, BL 
Citation: Christie DM, etal., Am J Physiol 1996 Aug;271(2 Pt 1):G377-85.
RGD ID: 729802
Pubmed: PMID:8770054   (View Abstract at PubMed)

An apical sodium-dependent bile acid transporter (ASBT) has recently been cloned and characterized in the rat ileum. Northern and Western blotting revealed both the ASBT mRNA and protein in rat kidney. The coding sequence of the kidney transcript was found to be identical to the previously cloned ileal ASBT. Indirect immunofluorescence studies localized the ASBT protein to the apical membrane of the renal proximal convoluted tubule. Kinetic analysis of sodium-dependent taurocholate uptake using membrane vesicles revealed a similar Michaelis-Menten constant value for taurocholate in the kidney and intestine. ASBT protein and function were present in the kidney but not the ileum from 7-day-old rats. On postnatal day 7, there was a sevenfold increase in ASBT steady-state mRNA levels in the kidney relative to the ileum, yet nuclear run-on assays revealed that the nascent transcription rates at this age were virtually the same. This suggests that the difference in the neonatal expression of the ASBT gene in the kidney and ileum may be in part due to differences in mRNA stability.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
bile acid and bile salt transport  IDA 729802 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane  IDA 729802 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
bile acid:sodium symporter activity  IDA 729802 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc10a2  (solute carrier family 10 member 2)


Additional Information