RGD Reference Report - Phospholipase C-gamma is required for agonist-induced Ca2+ entry. - Rat Genome Database

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Phospholipase C-gamma is required for agonist-induced Ca2+ entry.

Authors: Patterson, RL  Van Rossum, DB  Ford, DL  Hurt, KJ  Bae, SS  Suh, PG  Kurosaki, T  Snyder, SH  Gill, DL 
Citation: Patterson RL, etal., Cell 2002 Nov 15;111(4):529-41.
RGD ID: 729542
Pubmed: PMID:12437926   (View Abstract at PubMed)

We report here that PLC-gamma isoforms are required for agonist-induced Ca2+ entry (ACE). Overexpressed wild-type PLC-gamma1 or a lipase-inactive mutant PLC-gamma1 each augmented ACE in PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of PLC-gamma1 was ineffective. RNA interference to deplete either PLC-gamma1 or PLC-gamma2 in PC12 and A7r5 cells inhibited ACE. In DT40 B lymphocytes expressing only PLC-gamma2, overexpressed muscarinic M5 receptors (M5R) activated ACE. Using DT40 PLC-gamma2 knockout cells, M5R stimulation of ER Ca2+ store release was unaffected, but ACE was abolished. Normal ACE was restored by transient expression of PLC-gamma2 or a lipase-inactive PLC-gamma2 mutant. The results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium ion transport  IMP 729542 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Plcg1  (phospholipase C, gamma 1)


Additional Information