RGD Reference Report - Rat nurr1 is prominently expressed in perirhinal cortex, and differentially induced in the hippocampal dentate gyrus by electroconvulsive vs. kindled seizures.

General

Rat nurr1 is prominently expressed in perirhinal cortex, and differentially induced in the hippocampal dentate gyrus by electroconvulsive vs. kindled seizures.
Authors:	Xing, G Zhang, L Zhang, L Heynen, T Li, X L Smith, M A Weiss, S R Feldman, A N Detera-Wadleigh, S Chuang, D M Post, R M
Citation:	Xing G, etal., Brain Res Mol Brain Res 1997 Jul;47(1-2):251-61.
RGD ID:	729299
Pubmed:	PMID:9221923 (View Abstract at PubMed)
We isolated a rat orphan nuclear hormone receptor from a brain cortex cDNA library. The sequence of the cDNA insert was 2154 bp with an open reading frame of 1794 bp encoding a putative protein of 598 amino acids and predicted molecular mass of 65 kDa. The deduced amino acid sequence showed a strong homology to the mouse nurr1 and human NOT1 orphan nuclear hormone receptors of the NGFI-B/nur77/NAK1 gene subfamily. We refer to this rat clone as r-nurr1. Northern blot analysis showed that r-nurr1 mRNA was highly expressed in the brain and moderately in the lung as a 4.0 kb transcript. A smaller transcript of 2.5 kb was also detected in the testes. The level of r-nurr1 transcript in the heart, skeletal muscle, liver, kidney and spleen was marginal. In situ hybridization showed that r-nurr1 mRNA was constitutively expressed in various regions of the CNS, particularly in the deeper layers (IV to VI) of the perirhinal cortex and area 2 of parietal cortex. We further evaluated the modulation of r-nurr1 expression in CNS by an electroconvulsive seizure (ECS) and by an amgydala-kindled seizure. A single ECS administered via earclip electrodes induced a rapid and transient increase of r-nurr1 mRNA in the granule cells of the dentate gyrus, being significant at 15 min after the seizure, maximal approximately 1 h and back to baseline at 4 h. The amygdala kindled seizure revealed a less robust and restricted nurr-1 induction in the CNS, as only two of the four kindled animals showed a unilateral induction of nurr1 mRNA in the dentate gyrus. These results suggest that r-nurr1 is an immediate-early gene that is differentially induced by ECS vs. kindled seizures. In addition, as r-nurr1 is prominently expressed in the specific brain sites associated with memory acquisition and consolidation, it may play a role in memory processing.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
epilepsy		ISO	Nr4a2 (Rattus norvegicus)	729299; 729299	mRNA:increased expression:dentate gyrus	RGD
epilepsy		IEP		729299	mRNA:increased expression:dentate gyrus	RGD

Objects Annotated

Genes (Rattus norvegicus)

Nr4a2 (nuclear receptor subfamily 4, group A, member 2)

Genes (Mus musculus)

Nr4a2 (nuclear receptor subfamily 4, group A, member 2)

Genes (Homo sapiens)

NR4A2 (nuclear receptor subfamily 4 group A member 2)

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Rat nurr1 is prominently expressed in perirhinal cortex, and differentially induced in the hippocampal dentate gyrus by electroconvulsive vs. kindled seizures.