RGD Reference Report - Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain. - Rat Genome Database

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Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain.

Authors: Bond, CT  Ammala, C  Ashfield, R  Blair, TA  Gribble, F  Khan, RN  Lee, K  Proks, P  Rowe, IC  Sakura, H 
Citation: Bond CT, etal., FEBS Lett 1995 Jun 19;367(1):61-6.
RGD ID: 728997
Pubmed: PMID:7601286   (View Abstract at PubMed)

A cDNA clone encoding an inwardly-rectifying K-channel (BIR1) was isolated from insulinoma cells. The predicted amino acid sequence shares 72% identity with the cardiac ATP-sensitive K-channel rcKATP (KATP-1;[6]). The mRNA is expressed in the brain and insulinoma cells. Heterologous expression in Xenopus oocytes produced currents which were K(+)-selective, time-independent and showed inward rectification. The currents were blocked by external barium and caesium, but insensitive to tolbutamide and diazoxide. In inside-out patches, channel activity was not blocked by 1 mM internal ATP. The sequence homology with KATP-1 suggests that BIR1 is a subunit of a brain and beta-cell KATP channel. However, pharmacological differences and the lack of ATP-sensitivity, suggest that if, this is the case, heterologous subunits must exert strong modulatory influences on the native channel.

Objects referenced in this article
Gene Kcnj6 potassium inwardly-rectifying channel, subfamily J, member 6 Rattus norvegicus

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