RGD Reference Report - Regulated delivery of AMPA receptor subunits to the presynaptic membrane. - Rat Genome Database

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Regulated delivery of AMPA receptor subunits to the presynaptic membrane.

Authors: Schenk, U  Verderio, C  Benfenati, F  Matteoli, M 
Citation: Schenk U, etal., EMBO J 2003 Feb 3;22(3):558-68.
RGD ID: 728773
Pubmed: PMID:12554656   (View Abstract at PubMed)
PMCID: PMC140743   (View Article at PubMed Central)
DOI: DOI:10.1093/emboj/cdg059   (Journal Full-text)

In recent years, a role for AMPA receptors as modulators of presynaptic functions has emerged. We have investigated the presence of AMPA receptor subunits and the possible dynamic control of their surface exposure at the presynaptic membrane. We demonstrate that the AMPA receptor subunits GluR1 and GluR2 are expressed and organized in functional receptors in axonal growth cones of hippocampal neurons. AMPA receptors are actively internalized upon activation and recruited to the surface upon depolarization. Pretreatment of cultures with botulinum toxin E or tetanus toxin prevents the receptor insertion into the plasma membrane, whereas treatment with alpha-latrotoxin enhances the surface exposure of GluR2, both in growth cones of cultured neurons and in brain synaptosomes. Purification of small synaptic vesicles through controlled-pore glass chromatography, revealed that both GluR2 and GluR1, but not the GluR2 interacting protein GRIP, copurify with synaptic vesicles. These data indicate that, at steady state, a major pool of AMPA receptor subunits reside in synaptic vesicle membranes and can be recruited to the presynaptic membrane as functional receptors in response to depolarization.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
chemical synaptic transmission  IDA 728773 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
growth cone  IDA 728773 RGD 
presynaptic membrane  IDA 728773 RGD 
synaptic vesicle membrane  IDA 728773 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
AMPA glutamate receptor activity  TAS 728773 RGD 
glutamate-gated receptor activity  TAS 728773 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gria2  (glutamate ionotropic receptor AMPA type subunit 2)

Objects referenced in this article
Gene Gria1 glutamate ionotropic receptor AMPA type subunit 1 Rattus norvegicus

Additional Information