RGD Reference Report - Expression and distribution of glucose-6-phosphatase catalytic subunit messenger RNA and its changes in the diabetic state. - Rat Genome Database

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Expression and distribution of glucose-6-phosphatase catalytic subunit messenger RNA and its changes in the diabetic state.

Authors: Shingu, R  Nakajima, H  Horikawa, Y  Hamaguchi, T  Yamasaki, T  Miyagawa, J  Namba, M  Hanafusa, T  Matsuzawa, Y 
Citation: Shingu R, etal., Res Commun Mol Pathol Pharmacol 1996 Jul;93(1):13-24.
RGD ID: 728661
Pubmed: PMID:8865366   (View Abstract at PubMed)

Glucose-6-phosphatase (G6Pase) plays a major role in gluconeogenic pathway. To analyze its regulation, we have cloned a full-length cDNA for G6Pase catalytic subunit from the rat liver. In the cloned cDNA, a 492 base insertion, respective to the previously reported sequence, was found in the 3'-noncoding region. In both ends of this insertion, 5'- and 3' 2' splice site motifs were identified. However, spliced mRNA was hardly observed in the rat RNA. Abundant expression was observed in the liver and the kidney by Northern analysis. Expression was also observed in the spleen, adrenal gland and small intestines. Reverse-transcription (RT) polymerase chain reaction (PCR) analysis revealed that G6Pase mRNA was also expressed in a variety of tissues including pancreatic islets. Its expression was increased in the ketotic diabetic mice livers and was corrected by insulin treatment. However, no appreciable changes were observed in kidneys. Broad tissue distribution in expression and the tissue specificity in regulation were thus considered to be the important features of G6Pase gene.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
type 2 diabetes mellitus  ISOG6pc1 (Mus musculus)728661; 728661 RGD 
type 2 diabetes mellitus  IEP 728661 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
gluconeogenesis  TAS 728661 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glucose-6-phosphatase activity  TAS 728661 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
altered galactose metabolic pathway  ISS 728661 RGD 
altered galactose metabolic pathway  IEP 728661 RGD 
Objects Annotated

Genes (Rattus norvegicus)
G6pc1  (glucose-6-phosphatase catalytic subunit 1)

Genes (Mus musculus)
G6pc1  (glucose-6-phosphatase catalytic subunit 1)

Genes (Homo sapiens)
G6PC1  (glucose-6-phosphatase catalytic subunit 1)


Additional Information