RGD Reference Report - Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent diabetes mellitus.

General

Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent diabetes mellitus.
Authors:	Michelsen, BK Petersen, JS Boel, E Moldrup, A Dyrberg, T Madsen, OD
Citation:	Michelsen BK, etal., Proc Natl Acad Sci U S A 1991 Oct 1;88(19):8754-8.
RGD ID:	728511
Pubmed:	PMID:1924335 (View Abstract at PubMed)
PMCID:	PMC52588 (View Article at PubMed Central)
A 64-kDa islet protein is a major autoantigen in insulin-dependent diabetes mellitus (IDDM). Autoantibodies against the 64-kDa protein were recently shown to immunoprecipitate glutamic acid decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.1.15) from brain and from islets. We present evidence that the autoantisera also recognize a hydrophilic islet protein of approximately 67 kDa in addition to the amphiphilic 64-kDa form. We have isolated a full-length rat islet GAD cDNA encoding a hydrophilic 67-kDa protein, which appears to be identical to rat brain 67-kDa GAD. A partial sequence of human insulinoma 67-kDa GAD was identical to human brain 67-kDa GAD. Allelic variations were observed in rat as well as in human 67-kDa GAD sequences. The expressed rat islet 67-kDa GAD protein is functional and is immunoprecipitated by IDDM sera; it comigrates electrophoretically with the 67-kDa islet autoantigen. The hydrophilic 67-kDa form of GAD in islets is an additional autoantigen in IDDM and is recognized by a different subset of autoantibodies than the 64-kDa autoantigen. Thus, mammalian cell lines expressing functionally active, recombinant GAD may become important tools to study the nature and the role of GAD autoreactivity in IDDM.

RGD Manual Disease Annotations Click to see Annotation Detail View

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
diabetes mellitus		IDA		728511		RGD

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Biological Process

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
gamma-aminobutyric acid biosynthetic process	involved_in	IDA		728511	PMID:1924335	UniProt

Molecular Function

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
glutamate decarboxylase activity	enables	IDA		728511	PMID:1924335	UniProt
glutamate decarboxylase activity		IDA		728511		RGD

Objects Annotated

Genes (Rattus norvegicus)

Gad1 (glutamate decarboxylase 1)

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Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent diabetes mellitus.