RGD Reference Report - gamma-Aminobutyric acidA rho receptor subunits in the developing rat hippocampus. - Rat Genome Database

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gamma-Aminobutyric acidA rho receptor subunits in the developing rat hippocampus.

Authors: Didelon, F  Sciancalepore, M  Savic', N  Mladinic', M  Bradbury, A  Cherubini, E 
Citation: Didelon F, etal., J Neurosci Res 2002 Mar 15;67(6):739-44.
RGD ID: 728465
Pubmed: PMID:11891787   (View Abstract at PubMed)
DOI: DOI:10.1002/jnr.10178   (Journal Full-text)

The RT-PCR approach was used to estimate the expression of gamma-aminobutyric acid (GABA)(A) rho receptor subunits in the hippocampus of neonatal and adult rats. All three rho subunits were detected at postnatal day (P) 2, the rho3 subunit being expressed at an extremely low level. The rho1 and rho2 products appeared to be developmentally regulated; they were found to be more pronounced in adulthood. In another set of experiments, to correlate gene expression with receptor function, GABA(A) rho subunit mRNAs were detected with single-cell RT-PCR in CA3 pyramidal cells (from P3-P4 hippocampal slices), previously characterized with electrophysiological experiments for their bicuculline-sensitive or -insensitive responses to GABA. In 6 of 19 cells (31%), pressure application of GABA evoked at -70 mV inward currents that persisted in the presence of 100 microM bicuculline (314 plus minus 129 pA). RT-PCR performed in two of these neurons revealed the presence of rho1 and rho2 subunits, the latter being present with the alpha2 subunit. A rho2 subunit was also found in 1 neuron (among 9) exhibiting a response to GABA, which was completely abolished by bicuculline. This might be due to the lack of putative accessory GABA(A) subunits that can coassemble with rho2 to make functional receptors. Similar experiments from 10 P15 CA3 pyramidal cells failed to reveal any rho1-3 transcripts. However, these neurons abundantly express alpha3 subunits. It is likely that in CA3 pyramidal cells of neonatal and adult hippocampus GABA(A) rho subunits are present but at very low levels of expression.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
chloride channel activity  TAS 728465 RGD 
GABA-A receptor activity  IDA 728465 RGD 
inhibitory extracellular ligand-gated monoatomic ion channel activity  TAS 728465 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gabrr2  (gamma-aminobutyric acid type A receptor subunit rho 2)

Objects referenced in this article
Gene Gabrr1 gamma-aminobutyric acid type A receptor subunit rho 1 Rattus norvegicus

Additional Information