RGD Reference Report - Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor. - Rat Genome Database

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Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor.

Authors: Lin, HY  Kaji, EH  Winkel, GK  Ives, HE  Lodish, HF 
Citation: Lin HY, etal., Proc Natl Acad Sci U S A 1991 Apr 15;88(8):3185-9.
RGD ID: 728424
Pubmed: PMID:1849646   (View Abstract at PubMed)
PMCID: PMC51410   (View Article at PubMed Central)

By screening a cDNA library derived from the A10 rat vascular smooth muscle cell line for functional expression in COS cells, we have isolated a high-affinity receptor for endothelin 1 (Kd = 476 pM) and endothelin 2. The affinity of the cloned endothelin receptor for endothelin 3 is greater than 100 times less in A10 cells and in a CHO cell line stably transformed by the endothelin receptor cDNA. The 426-amino acid receptor polypeptide has seven putative hydrophobic transmembrane domains and is presumed to be a member of the family of guanine nucleotide-binding regulatory (G) protein-coupled receptors. Microinjection of in vitro transcripts of the cloned cDNA into CHO cells confers a transient increase in intracellular calcium in response to endothelin 1, indicating that the receptor is functional and couples to the appropriate G protein(s). RNA analysis reveals high expression in rat lung and heart, tissues known to exhibit binding to iodinated endothelin 1.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cytosolic calcium ion concentration  IDA 728424 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endothelin receptor activity  IDA 728424 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ednra  (endothelin receptor type A)


Additional Information