RGD Reference Report - Expression of angiogenic factors in rat periapical lesions. - Rat Genome Database

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Expression of angiogenic factors in rat periapical lesions.

Authors: Yamanaka, Y  Kaneko, T  Yoshiba, K  Kaneko, R  Yoshiba, N  Shigetani, Y  Nor, JE  Okiji, T 
Citation: Yamanaka Y, etal., J Endod. 2012 Mar;38(3):313-7. doi: 10.1016/j.joen.2011.11.009. Epub 2011 Dec 23.
RGD ID: 7257692
Pubmed: PMID:22341067   (View Abstract at PubMed)
DOI: DOI:10.1016/j.joen.2011.11.009   (Journal Full-text)

INTRODUCTION: Angiogenic factors such as VEGFR2 (vascular endothelial cell growth factor receptor 2), Bcl-2 (a prosurvival and proangiogenic signaling molecule), and chemokine (C-X-C motif) ligand 1 (CXCL1) (a proangiogenic chemokine) may have critical roles in enhancing the establishment of apical periodontitis. To understand the role of these factors in the pathogenesis of apical periodontitis, we conducted immunohistochemical and molecular biological analysis. METHODS: Apical periodontitis was induced in the lower first molars of Wistar rats by making unsealed pulp exposures. After, 14, 21, and 28 days, the molars were retrieved, embedded as frozen sample blocks, and cut in a cryostat. Normal lower first molars served as controls. Immunostaining for CD31 (a marker for endothelial cells), Bcl-2, and real-time polymerase chain reaction analysis of VEGFR2, Bcl-2, CXCL1, and CXCR2 messenger RNA were performed. In the real-time polymerase chain reaction analysis, messenger RNA was extracted from CD31-stained endothelial cells that were retrieved with laser capture microdissection. For statistical analysis of immunohistochemistry, the immunostained area was plotted, and pixel counts were determined. Then, the percentage of the immunostained area in the total area was calculated. RESULTS: The density of the CD31-stained area increased until 21 days after pulp exposure. On the other hand, Bcl-2-stained area showed the highest density at 14 days (active lesion expanding phase) and then decreased until 28 days (lesion stability phase). VEGFR2, Bcl-2, CXCL1, and CXCR2 messenger RNA expression in endothelial cells showed the highest levels at 14 days and then decreased until 28 days. CONCLUSIONS: The increase in microvascular density and the up-regulation of VEGFR2, Bcl-2, CXCL1, and CXCR2 messenger RNA expression in endothelial cells took place coincidently with the expanding phase of experimentally induced periapical lesions. These data suggest that these angiogenic factors play a role in the lesion development.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
periapical periodontitis  ISOCxcr2 (Rattus norvegicus)7257692; 7257692 RGD 
periapical periodontitis  IEP 7257692 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcr2  (C-X-C motif chemokine receptor 2)

Genes (Mus musculus)
Cxcr2  (C-X-C motif chemokine receptor 2)

Genes (Homo sapiens)
CXCR2  (C-X-C motif chemokine receptor 2)


Additional Information