RGD Reference Report - mRNA differential display analysis of nephrotic kidney glomeruli. - Rat Genome Database

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mRNA differential display analysis of nephrotic kidney glomeruli.

Authors: Haltia, A  Solin, M  Luimula, P  Kretzler, M  Holthofer, H 
Citation: Haltia A, etal., Exp Nephrol. 1999 Jan-Feb;7(1):52-8.
RGD ID: 7248412
Pubmed: PMID:9892814   (View Abstract at PubMed)
DOI: DOI:20584   (Journal Full-text)

BACKGROUND: Differential display RT-PCR (DDRT-PCR) is a new powerful technique for identification and characterization of altered gene expression in eukaryotic cells and tissues. We studied here changes in kidney glomerular gene expression in patients with congenital nephrotic syndrome of the Finnish type (CNF), an inherited kidney disease with heavy proteinuria already in utero. METHODS: Using the DDRT-PCR approach and isolated glomeruli from removed human kidneys, we compared the gene expression patterns of normal human and CNF glomeruli. Differential expression of candidate genes was verified by Northern blotting, and the corresponding PCR fragments were sequenced and compared to known sequences in databanks. RESULTS: We found several genes and sequence tags with altered expression in nephrotic glomeruli including fragments with close homologies to cytochrome c oxidase subunit I, integrin-linked kinase, insulin-like growth factor II receptor and eotaxin, and also clones resembling anchyrin and cadherin-like consensus sequences. CONCLUSION: All the sequences identified are of interest in respect to pathogenesis of proteinuria. Furthermore, this study reveals potentially new members to known gene families with tissue and cell type-specific expression.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nephrotic syndrome type 1  IEP 7248412 RGD 
nephrotic syndrome type 1  ISOCCL11 (Homo sapiens)7248412; 7248412 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccl11  (C-C motif chemokine ligand 11)

Genes (Mus musculus)
Ccl11  (C-C motif chemokine ligand 11)

Genes (Homo sapiens)
CCL11  (C-C motif chemokine ligand 11)


Additional Information