RGD Reference Report - Molecular cloning of rat phosphatidylinositol synthase cDNA by functional complementation of the yeast Saccharomyces cerevisiae pis mutation. - Rat Genome Database

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Molecular cloning of rat phosphatidylinositol synthase cDNA by functional complementation of the yeast Saccharomyces cerevisiae pis mutation.

Authors: Tanaka, S  Nikawa, J  Imai, H  Yamashita, S  Hosaka, K 
Citation: Tanaka S, etal., FEBS Lett 1996 Sep 9;393(1):89-92.
RGD ID: 724663
Pubmed: PMID:8804431   (View Abstract at PubMed)

Phosphatidylinositol synthase (CDP-1,2-diacyl-sn-glycerol: 3-phosphatidyltransferase, EC 2.7.8.11) catalyzes the formation of phosphatidylinositol and CMP from CDP-diacylglycerol and myo-inositol. We have cloned a phosphatidylinositol synthase cDNA from rat brain by functional complementation of the yeast pis mutation, which is defective in phosphatidylinositol synthase. The deduced protein comprised 213 amino acids with a calculated molecular mass of 23,613 Da. The predicted protein sequence is highly homologous to the previously determined yeast phosphatidylinositol synthase sequence. The cDNA hybridized to a 1.7-kb mRNA that was abundantly expressed in rat brain and kidney.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
phosphatidylinositol biosynthetic process  IDA 724663 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cdipt  (CDP-diacylglycerol--inositol 3-phosphatidyltransferase)


Additional Information