RGD Reference Report - Modulation of RAGE isoforms expression in the brain and plasma of rats exposed to transient focal cerebral ischemia. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Modulation of RAGE isoforms expression in the brain and plasma of rats exposed to transient focal cerebral ischemia.

Authors: Greco, R  Amantea, D  Mangione, AS  Petrelli, F  Gentile, R  Nappi, G  Blandini, F  Corasaniti, MT  Tassorelli, C 
Citation: Greco R, etal., Neurochem Res. 2012 Jul;37(7):1508-16. doi: 10.1007/s11064-012-0778-1. Epub 2012 Apr 19.
RGD ID: 7245542
Pubmed: PMID:22528836   (View Abstract at PubMed)
DOI: DOI:10.1007/s11064-012-0778-1   (Journal Full-text)

Activation of RAGE (receptor for advanced glycation endproducts) and of its subtypes may play a role in neuronal damage and neuroinflammation associated with brain ischemia, though the underlying mechanisms remain unclear. In this study, we have examined by Western blotting the expression of RAGE isoforms in the cerebral cortex and striatum of Wistar rats subjected to transient (1 or 2 h) middle cerebral artery occlusion (tMCAo). The findings show that the full-length RAGE (~50 kDa) and its isoforms in the 26-43 kDa range are significantly decreased in the ischemic cortex, but not in the striatum, after 1 and 2 h tMCAo when compared to the sham group. By contrast, in the striatum, ischemia-reperfusion injury caused a significant increase of full-length RAGE and its isoforms in the 72-100 kDa range. We also investigated the soluble form of RAGE, which was significantly decreased in the plasma of rats subjected to transient or permanent MCAo. In conclusion, the present data demonstrate that regional brain expression of RAGE is differentially affected by tMCAo in rat. These modifications are accompanied by a decrease in the plasma levels of soluble RAGE, thereby suggesting a potential role for soluble RAGE as a peripheral biomarker of focal ischemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
transient cerebral ischemia  ISOAger (Rattus norvegicus)7245542; 7245542protein:altered expression:cerebral cortex and striatum (rat)RGD 
transient cerebral ischemia  IEP 7245542protein:altered expression:cerebral cortex and striatum (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ager  (advanced glycosylation end product-specific receptor)

Genes (Mus musculus)
Ager  (advanced glycosylation end product-specific receptor)

Genes (Homo sapiens)
AGER  (advanced glycosylation end-product specific receptor)


Additional Information