RGD Reference Report - Diabetes and radiocontrast media increase endothelin converting enzyme-1 in the kidney. - Rat Genome Database

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Diabetes and radiocontrast media increase endothelin converting enzyme-1 in the kidney.

Authors: Khamaisi, M  Raz, I  Shilo, V  Shina, A  Rosenberger, C  Dahan, R  Abassi, Z  Meidan, R  Lecht, S  Heyman, SN 
Citation: Khamaisi M, etal., Kidney Int. 2008 Jul;74(1):91-100. doi: 10.1038/ki.2008.112. Epub 2008 Apr 2.
RGD ID: 7243946
Pubmed: PMID:18385664   (View Abstract at PubMed)
DOI: DOI:10.1038/ki.2008.112   (Journal Full-text)

Plasma endothelin-1 levels rise in diabetes and after exposure to contrast media suggesting a role in progressive diabetic and acute radiocontrast nephropathies. Here we studied individual and combined effects of streptozotocin-induced diabetes and contrast media on renal endothelin converting enzyme-1 levels in the rat. In vivo, medullary (but not cortical) endothelin converting enzyme protein gradually increased 4 to 5-fold following the induction of diabetes or after the administration of contrast media but rose 15-fold when diabetic rats were given contrast media. Changes in mRNA expression paralleled those of the protein. Immunohistochemistry confirmed that increased tubular and endothelial cell endothelin converting enzyme-1 were most pronounced in the medulla. In vitro, endothelin-1 levels increased 3-fold following incubation of endothelial cells with media high in glucose or with contrast and 4-fold with their combination. Endothelin converting enzyme-1 protein and mRNA expression changed in a similar pattern while prepro endothelin-1 mRNA increased with each insult but not in an additive way. Our study shows that diabetes and contrast media up-regulate renal medullary endothelin converting enzyme-1 expression and synthesis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Contrast-Induced Nephropathy  ISOEce1 (Rattus norvegicus)7243946; 7243946mRNA and protein:increased expression:kidney medullaRGD 
Contrast-Induced Nephropathy  IEP 7243946mRNA and protein:increased expression:kidney medullaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ece1  (endothelin converting enzyme 1)

Genes (Mus musculus)
Ece1  (endothelin converting enzyme 1)

Genes (Homo sapiens)
ECE1  (endothelin converting enzyme 1)


Additional Information